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    Role and regulatory mechanism of type 2 immunity in tissue repair
    GE Shaohua, DING Tian, LIU Hongrui
    Journal of Shandong University (Health Sciences)    2021, 59 (9): 51-56.   DOI: 10.6040/j.issn.1671-7554.0.2021.0961
    Abstract2364)   HTML46)    PDF(pc) (1417KB)(1289)       Save
    The repair and regeneration of damaged tissue has always been a research hotspot in regenerative medicine. Traditional regenerative medicine relies on stem cell, growth factor, and scaffold to achieve tissue regeneration, but the safety and effectiveness in the clinic still need to be verified. In recent years, growing evidences have shown that the immune system is crucial in tissue repair and regeneration, which provides a new avenue for regenerative medicine. Type 2 immunity, involved in various immune cell subsets, is characterized by the production of interleukin(IL)-4, IL-5, IL-9, IL-10, IL-13, and IL-33. It plays a key role in regulating the immune environment during tissue repair. This paper reviews the role and regulatory mechanism of type 2 cytokines and individual immune cell subsets in tissue repair and regeneration following injury.
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    Association between platelet count and metabolic syndrome based on a prospective cohort study
    MA Xiaotian, GU Jianhua, WANG Li, XUE Fuzhong, LIU Yanxun
    Journal of Shandong University (Health Sciences)    2018, 56 (12): 92-97.   DOI: 10.6040/j.issn.1671-7554.0.2018.411
    Abstract718)      PDF(pc) (1016KB)(306)       Save
    Objective To explore the correlation between platelet count and metabolic syndrome. Methods Our cohort was based on a “Multi-center Health Management Cohort”. People who did at least two health examinations, didnt have missing value in important variables, and were free of metabolic syndrome at baseline were selected into the prospective cohort. The study subjects were divided into four groups according to the baseline platelet count quartile, and the incidence density of each group was compared. The characteristics of each variable were described in the four groups at baseline. The Cox proportional regression model was used in this study, where we adjusted age, gender, BMI, hyperglycemia, hypertension and dysplasia gradually, to explore whether the platelet count was still a risk factor for metabolic syndrome before and after adjustment of confounding factors. Results There were 14 173 individuals who were aged 21-60 in this cohort. The total follow-up time was 41 014.8 person-year. The average follow-up time was 2.89 years. A total of 1 611 people were diagnosed as having metabolic syndrome. The incidence density of this cohort was 39.28/1 000 person-year. The platelet counts were always statistically significant in model 1(single factor analysis), 山 东 大 学 学 报 (医 学 版)56卷12期 -马晓天,等.血小板计数与代谢综合征关联性的前瞻性队列研究 \=-model 2(adjusted for age and gender)and model 3(adjusted for age, gender, BMI, hyperglycemia, hypertension and dysplasia), with similar HR values. Q2 didnt have a statistical difference with Q1 in model 1, while Q3 and Q4 had. In model 2 and model 3, Q2-Q4 all had statistical significance when compared to Q1, and their HR values increased from Q2 to Q4. It showed that with the increase of platelet count, the risk of metabolic synthesis increased. Conclusion Elevated platelet count is an independent risk factor for metabolic syndrome.
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    A retrospective analysis of chromosome karyotypes in RSA couples from assisted reproductive population
    ZHAO Bingqing, GAO Xuan, LI Jiangxia
    Journal of Shandong University (Health Sciences)    2021, 59 (7): 26-31.   DOI: 10.6040/j.issn.1671-7554.0.2021.0102
    Abstract874)      PDF(pc) (828KB)(205)       Save
    Objective To analyze the peripheral blood chromosome karyotypes of patients assisted with reproductive technology, so as to provide reference for the clinical diagnosis and treatment and genetic counseling. Methods From Jan. 2015 to Dec. 2018, 1 438(719 couples)patients with recurrent spontaneous abortion(RSA)and 3 292(1 646 couples)patients with one-time spontaneous abortion undergoing assisted reproduction were selected for chromosome karyotype analysis. The types, incidences and ratios of chromosome variations between males and females were compared. Relationship between the number of spontaneous abortions and chromosome karyotypes was analyzed. Results Of the 1 438(719 couples)RSA patients, 320 chromosome abnormalities were detected, among which 165 were male cases and 155 were female cases. Chromosomal structural aberrations accounted for 57.58% of chromosomal abnormalities. The ratio of mutual translocations was the highest. For the number of abortions, significant differences were detected in chromosome reciprocal translocation and Robertsonian translocation between one-time spontaneous abortion group and RSA group(P<0.01), but no significant difference in chromosome inversion was observed(male P=0.21, female P=0.83). As the number of abortions increased, the trends of male and female chromosome aberration were statistically significant(P<0.01). Conclusion The rate of chromosomal abnormality in patients with one-time spontaneous abortion is lower than that in RSA patients. The most common chromosomal abnormality in RSA patients is chromosomal translocation, and the rate of chromosomal abnormality is not related to the number of abortions.
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    Effect of double-lumen-tube or bronchial blocker used for one-lung ventilation on PetCO2 and PaCO2
    YANG Xiaomei, FANG Ningning, YI Wenbo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2015, 53 (11): 77-79.   DOI: 10.6040/j.issn.1671-7554.0.2015.262
    Abstract951)      PDF(pc) (1526KB)(1352)       Save
    Objective To assess the effect of double-lumen-tube (DLT) and bronchial blocker for one-lung ventilation on partial pressure of end-tidal carbon dioxide (PetCO2) and arterial partial pressure of carbon dioxide (PaCO2). Methods Thirty patients were randomly assigned to DLT group (n=15) and BB group (n=15). After anesthesia induction, DLT or BB was intubated into the trachea. After one-lung ventilation, the values of PetCO2 and PaCO2 were recorded and analyzed. Results Compared with BB group, the PetCO2 value was higher, the PaCO2 value was lower, and the correlation between PetCO2 and PaCO2 was better in DLT group. Conclusion Compared with BB, DLT was better at exhalation of CO2.
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    Construction and significance of a rat model of OSAHS with chronic intermittent hypoxia
    WANG Haipeng, ZOU Juanjuan, GAO Chunmiao, WANG Xiao, WANG Yan, LI Yanzhong
    Journal of Shandong University (Health Sciences)    2021, 59 (2): 7-13.   DOI: 10.6040/j.issn.1671-7554.0.2020.1469
    Abstract1346)      PDF(pc) (6963KB)(417)       Save
    Objective To construct a rat model of obstructive sleep apnea hypopnea syndrome(OSAHS)with chronic intermittent hypoxia. Methods A color touch screen, a programmable logic controller(PLC)automatic control, and an automated data acquisition and monitoring system were used to assemble the equipment needed to construct the experimental model. The hypoxic chamber was composed of the main engine, the hypoxic chamber and the gas path control. A total of 30 rats were randomly divided into two groups, normoxic control(CON)group and CIH model(CIH). After 9 weeks of normal feeding, the rats were sacrificed, and liver samples were collected for hematoxylin and eosin(HE)staining, transmission electron microscopy, and Oil Red “O” staining. Results Morphological changes: HE staining of the CIH group showed lipid accumulation and lipid vacuole formation leading to hepatocyte steatosis. Microscopy of the ultrastructure showed large clusters of fat droplets and appearance of large numbers of autophagosomes and lysosomes, focal accumulation of glycogen in the rough endoplasmic reticulum, and sparse microvilli in the bile canaliculi. these were rarely seen in the CON group. The CIH group showed obvious morphological changes. Oil Red O staining results: the difference in IOD/Area was statistically significant between the CIH group and CON group(The test statistic=-56.308, P=0.001). Hepatocytes in the CIH group were obviously stained red. Conclusion The rat model of OSAHS with chronic intermittent hypoxia is successful, reliable and effective, which is consistent with the pathophysiological characteristics of OSAHS, and lays a solid foundation for further research on the pathogenesis of abnormal function of various tissues and organs in rats and the possible therapeutic effects of related drugs.
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    Latest research advances on factors that influence endometrial receptivity
    CHEN Zijiang, YAN Junhao
    Journal of Shandong University (Health Sciences)    2021, 59 (8): 1-7.   DOI: 10.6040/j.issn.1671-7554.0.2021.0861
    Abstract1670)   HTML62)    PDF(pc) (1430KB)(929)       Save
    Good endometrial condition is essential to successful pregnancy. Endometrial receptivity, the capability of endometrium to receive embryo, will improve during the window of implantation in menstruation cycle, indicating the accurate time for implantation. As reported, 2/3 cases on embryo implantation failure or pregnancy loss were attributed to endometrial dysfunction. Evidence based on studies about bad endometrial receptivity and exact mechanism thereof, will provide effective approaches for diagnosis and treatment of these dysfunctions. In this review, we elaborated multiple underlying factors that influence endometrial receptivity, hoping to provide reference for further studies on pathophysiological mechanisms of endometrium in embryo implantation failure or pregnancy loss.
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    Frontier research hotspot and progress in assisted reproductive technology
    SHI Yuhua, WANG Qiumin, QI Dan
    Journal of Shandong University (Health Sciences)    2021, 59 (9): 97-102.   DOI: 10.6040/j.issn.1671-7554.0.2021.0826
    Abstract2896)   HTML107)    PDF(pc) (1425KB)(1479)       Save
    Assisted reproductive technology(ART), as an emerging discipline, has developed rapidly in recent decades, and has continuously made breakthroughs in the field of human reproductive health. The development and application of new technologies should be based on laws and ethics, with safety as the prerequisite, and continuously improve the effectiveness of the technology. In recent years, in order to improve the level of reproductive health and quality of newborn population, preimplantation genetic testing(PGT), mitochondrial replacement(MRT), freeze-thaw embryo transfer(FET)and cell therapy have become the focus of reproductive experts. This paper analyzes the research progress on hot issues in the field of ART.
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    Observation on “five no” behaviors of 316 children with language development retardation
    GUO Lingling, CHE Chao, CHEN Kunping, CHAI Runyu, YANG Bingshang, CAO Aihua
    Journal of Shandong University (Health Sciences)    2021, 59 (2): 95-101.   DOI: 10.6040/j.issn.1671-7554.0.2020.1367
    Abstract1501)      PDF(pc) (1545KB)(665)       Save
    Objective To explore the application of “five no” behaviors in language clinic, including no(less)seeing, no(less)responding, no(less)referring, no(less)language and inappropriateness. Methods The clinical data of 316 children complaining of “language retardation” in the Department of Child Health and Developmental Behavior, Qilu Hospital of Shandong University were reviewed. According to the DSM-5 diagnosis, the children were divided into autism spectrum disorder(ASD)group, general growth retardation(GDD)group and language disorder(LD)group. The “five no” behaviors observed at the first visit were compared among the three groups. Correlation analysis and Logistic regression analysis were conducted between the “five no” behaviors and scores of autism behavior scale(ABC), childhood autism rating scale(CARS), symbolic play ability test(SPT), and Gesell developmental diagnosis scale(GDS). Results There were statistically significant differences in no(less)seeing, no(less)responding, no(less)referring, and inappropriateness between ASD group and GDD group, LD group(P<0.001). There was a positive correlation between “five no” behaviors score with ABC and CARS scores(P<0.001), and a moderate negative correlation with SPT and GDS in coping with human ability, material ability and speech ability(P<0.001). Regression analysis showed that younger children with lower score of coping ability and higher score of CARS had higher score of “five no” behaviors(P<0.05). Conclusion By analyzing the characteristics of “five no” behaviors in ASD, GDD and LD children, we found that examining “five no” behaviors is an effective method to identify children with language development retardation in language clinic.
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    Application and problems of laparoscopic in assisted reproductive therapy
    HAN Ting, CHEN Honglei
    Journal of Shandong University (Health Sciences)    2019, 57 (10): 45-51.   DOI: 10.6040/j.issn.1671-7554.0.2019.754
    Abstract361)      PDF(pc) (1221KB)(326)       Save
    With the continuous development of assisted reproductive technology and the significant increase of pregnancy rate, there are still many difficult problems, such as hydrosalpinx, endometriosis, and uterine myoma. These diseases affect the success rate of assisted reproductive therapy and produce many troublesome complications, such as ectopic 山 东 大 学 学 报 (医 学 版)57卷10期 -韩婷,等.辅助生殖治疗中腹腔镜手术应用及注意问题 \=-pregnancy and ovarian torsion, which often require laparoscopic surgery. This article reviews the application and attention problems of laparoscopic in assisted reproductive therapy in order to provide reference for the clinical treatment of infertile patients.
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    Evaluation of learning satisfaction of preventive medicine students with Health Statistics
    HAN Xuan, SUN Xiubin, LIU Yi, WANG Shukang, LIU Jing, YUAN Zhongshang, ZHANG Tao, LI Xiujun, XUE Fuzhong, LIU Yunxia
    Journal of Shandong University (Health Sciences)    2022, 60 (3): 109-113.   DOI: 10.6040/j.issn.1671-7554.0.2020.1130
    Abstract710)      PDF(pc) (1642KB)(486)       Save
    Objective To investigate the learning satisfaction of preventive medicine students with the course of Health Statistics, and to explore the influencing factors, so as to provide reference for optimizing teaching design and teaching effects. Methods A self-designed questionnaire was distributed on the Wenjuanxing platform among undergraduates of two grades to collect their satisfaction with Health Statistics and the possible influencing factors. Data collected were analyzed with rank sum test, Chi-square test and multiple linear regression. Results A total of 190 effective questionnaires(97.44%)were collected. The score of learning satisfaction was 83, and the scores of learning motivation, learning environment, teacher-student interaction and teaching design were 16, 20, 20 and 24 respectively. Multiple linear regression analysis showed that student learning score rank, learning motivation, teacher-student interaction and teaching design had an impact on learning satisfaction. Conclusion Preventive medicine students have high satisfaction with Health Statistics. Future teaching should stimulate students learning motivation, increase the effective interaction between teachers and students, and optimize the teaching design, so as to improve students satisfaction and improve the overall teaching quality.
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    Contents and ratios of type I and type III collagens in normal skin and hypertrophic scar in people of different ages
    WANG Cheng, RONG Yanhua, SHEN Yuming, ZHANG Guoan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2016, 54 (11): 64-67.   DOI: 10.6040/j.issn.1671-7554.0.2015.1043
    Abstract2855)      PDF(pc) (1915KB)(1283)       Save
    Objective To quantify the contents of type I and III collagens and their ratios in normal skin and hypertrophic scar in people of different ages, and to explore the rule and cause of changes. Methods Normal human skin specimens and hypertrophic scar specimens were obtained from spontaneously aborted fetus and people of different ages, including the children and adolescent group(≤18 years), adult group(19-50 years), elderly group(>50 years). The total collagen contents were determined by hydroxyproline method. The ratios of types I and III collagens were examined by immunohistochemistry, and their contents were calculated. Results The difference of the contents of types I and III collagens and their ratios in normal skin were statistically significant among different age groups(P=0.01). Their ratios increased with the increase of age, and the differences were statistically significant among different age groups(P=0.02). The difference of contents of types I and III collagens and their ratios in hypertrophic scars were statistically significant among all groups, also between any two groups(P=0.03). In every age group, the difference of the contents of types I and III collagens and their ratios between normal skin and hypertrophic scar was statistically significant (P=0.02). Conclusion With age increasing, the changes of the ratios of types I and III collagens in the normal skin changes, which results in changes of the tension and elasticity of skin and the healing process after injury. The contents of types I and III collagens and their ratios significantly changes in hypertrophic scars, resulting in different structure of scar from normal skin.
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    Fertility preservation in patients with endometriosis
    YAN Lei, YUE Caixin, LIU Yichun
    Journal of Shandong University (Health Sciences)    2022, 60 (9): 31-34.   DOI: 10.6040/j.issn.1671-7554.0.2022.0843
    Abstract735)   HTML21)    PDF(pc) (1343KB)(391)       Save
    Endometriosis can affect female fertility by reducing ovarian reserve, changing the immune system and pelvic anatomical structure, and reducing endometrial receptivity. Therefore, fertility should be protected and preserved in patients with endometriosis. For such patients, early diagnosis, standardized treatment and long-term management can help to protect fertility. In addition, measures such as embryo freezing, mature oocyte freezing and ovarian tissue freezing, can help to preserve fertility. Fertility protection requires a multidisciplinary consultation mechanism.
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    A general overview of neuromodulation
    Di ZHANG,Meng YU,Xia LIU
    Journal of Shandong University (Health Sciences)    2020, 1 (8): 50-60.   DOI: 10.6040/j.issn.1671-7554.0.2020.0602
    Abstract4147)   HTML164)    PDF(pc) (4269KB)(1432)       Save

    Neuromodulation refers to a series of biomedical engineering techniques which normalize or modulate the function of the nervous system through invasive or non-invasive delivery of physical or chemical stimulus. In the past thirty years, with the in-depth understanding of the nervous system and its mechanisms of action, as well as the rapidly developing modern techniques, neuromodulation has evolved from basic concept to clinical applications. At the same time, new concepts and alternative methods of neuromodulation are constantly being innovated, tested and put into practice. The advancing of neuromodulation techniques will not only provide new tools for basic neuroscience research but also offer novel therapeutic options for neurological and psychiatric disorders. In this overview, we will briefly introduce the current status of different types of neuromodulation techniques, including the clinical applications, underlying mechanisms of their therapeutic effects and advantages/disadvantages, as well as the prospects for the future.

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    Effect of vitamin B on hyperhomocysteinemia in renal-transplant recipients
    ZHAO Jing-jie,LIU Jun-li,TIAN Chuan,ZHANG Cai
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract2040)      PDF(pc) (250KB)(693)       Save
    Objective: To explore the effect of folic acid, Vit B6, and Vit B12 on hyperhomocysteinemia in renal-transplant recipients. Methods: 195 renal-transplant recipients were randomly divided into the vitamin treatment group (group A, n=103, folic acid 5mg/d, vitamin B6 50mg/d, Bl2 1mg/d, totally 2 months) and the control group (group B, n=92). The plasma Hcy levels were determined before treatment, before operation and 1, 10, 20 and 30 days after operation. Results: The plasma level of Hcy in group B was significantly higher than that in group A before and after operation. The rate of acute rejection was lower in group A than in group B. Conclusion: Vitamin B supplementation can reduce the homocysteine level and the rate of acute rejection for renal-transplant recipients.
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    Role of cholesterol metabolism in osteoarthritis: a review of research progresses
    ZHAO Zhibo, MAN Zhentao, LI Wei
    Journal of Shandong University (Health Sciences)    2024, 62 (2): 1-9.   DOI: 10.6040/j.issn.1671-7554.0.2024.0045
    Abstract85)      PDF(pc) (4406KB)(82)       Save
    Osteoarthritis(OA)is a degenerative disease associated with aging and has long been linked to obesity. Some studies suggest that OA might be a metabolic disease, impacting not only weight-bearing joints but also non-weight-bearing joints. Emerging evidence indicates abnormal cholesterol metabolism in osteoarthritis, with pathways leading to the production of pro-inflammatory substances crucial to OA development. Drugs capable of modulating abnormal cholesterol metabolism hold considerable therapeutic promise for OA. This article aims to summarize the role and comprehensive molecular mechanisms of cholesterol metabolism in osteoarthritis and proposes that a multi-faceted regulation of cholesterol metabolism could be a promising strategy for the clinical treatment of osteoarthritis.
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    Locmotion control of quadruped robot based on deep reinforcement learning: review and prospect
    Wei ZHANG,Wenhao TAN,Yibin LI
    Journal of Shandong University (Health Sciences)    2020, 1 (8): 61-66.   DOI: 10.6040/j.issn.1671-7554.0.2020.0607
    Abstract2769)   HTML81)    PDF(pc) (1128KB)(827)       Save

    Brain-inspired deep reinforcement learning has recently led to a wide range of successes in different domains such as artificial intelligence and robotics. The method combining both advantages of deep learning and reinforcement learning gets strong capability of perception and decision-making. In this paper, we first provide a brief overview of two kinds of widely used deep reinforcement learning method and their fundamentals, then introduce the current status of deep reinforcement learning applying on quadruped robots. Finally, by summarizing the existing methods and the characteristics of locomotion for quadruped robots, we present future potential of deep reinforcement learning on quadruped robots.

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    SARS-CoV-2 infection, innate immunity and inflammatory response
    Xi ZHOU,Muhan HUANG,Yujie REN,Yang QIU
    Journal of Shandong University (Health Sciences)    2021, 59 (5): 15-21.   DOI: 10.6040/j.issn.1671-7554.0.2021.0368
    Abstract3190)   HTML188)    PDF(pc) (1512KB)(954)       Save

    Coronavirus disease 2019 (COVID-19), which is caused by a novel coronavirus named severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has become the worst pandemic and public health crisis across the globe once a century. This pandemic has caused huge losses in both human lives and global economy. Innate immunity is the first line of defense against pathogenic invasions. Extensive studies by scientists in China and the world have reported that SARS-CoV-2 can employ multiple strategies to evade host innate immunity, and such immune evasion mechanisms have become critical contributing factors for the pathogenicity of SARS-CoV-2. On the other hand, the pathogenesis of COVID-19 has been found to be closely relevant with the pro-inflammatory responses induced by SARS-CoV-2 infection in humans. This paper provides a brief review to the relationship between SARS-CoV-2 infection and innate immunity as well as inflammation.

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    Status and progress of oocyte cryopreservation
    MA Xiang, ZHAO Feiyan
    Journal of Shandong University (Health Sciences)    2022, 60 (9): 19-23.   DOI: 10.6040/j.issn.1671-7554.0.2022.0874
    Abstract638)   HTML16)    PDF(pc) (1233KB)(262)       Save
    Oocyte cryopreservation has a history of more than 30 years, which is mainly applicable to the fertility preservation of cancer patients before radiotherapy and chemotherapy, or non-cancer patients and people who delay childbearing due to personal factors. In addition, it can also be used as a remedy for male inability to provide sperm due to special factors in the process of assisted reproduction. At present, the commonly used methods of oocyte cryopreservation include slow cryopreservation and vitrification cryopreservation, and with the progress of research, vitrification has gradually replaced slow cryopreservation. Many studies have shown no significant differences in clinical pregnancy rates, obstetric and perinatal outcomes between embryos transferred using frozen eggs and fresh eggs.
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    Concerns and perspectives for multigene tests of breast cancer: opportunities and challenges
    YU Zhigang, WANG Fei
    Journal of Shandong University (Health Sciences)    2018, 56 (1): 22-26.   DOI: 10.6040/j.issn.1671-7554.0.2017.1036
    Abstract469)      PDF(pc) (1430KB)(338)       Save
    Both Oncotype DX® and MammaPrint® have been recommended by American Society of Clinical Onco-logy(ASCO)to guide decisions on adjuvant systemic therapy for women with early-stage invasive breast cancer. However, based on current evidence, multigene tests are still regarded supplementary to traditional clinical pathological examinations, and confined to specific patients. Besides, there are still clinical challenges concerning multigene tests: slow concordance of risk assignment by different tests, inaccurate prediction of late recurrences and poor prediction of drug-specific or regimen-specific benefits. In the future, multi-omics characteristic spectrum based on high-throughput technology platforms, innovative prediction models based on interdisciplinary research, and dynamic risk monitoring based on repetitive tests, will offer new opportunities and directions for multigene tests of breast cancer.
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    Research advances on the immunological pathogenesis and immunotherapy of premature ovarian failure
    QIN Yingying, ZHANG Xiruo
    Journal of Shandong University (Health Sciences)    2018, 56 (4): 33-37.   DOI: 10.6040/j.issn.1671-7554.0.2017.1280
    Abstract523)      PDF(pc) (1364KB)(318)       Save
    Premature ovarian failure(POF)is characterized by cessation of ovarian function in women younger than 40 years. It presents highly heterogeneous clinical phenotype and etiology, and greatly jeopardizes the fertility of patients. 山 东 大 学 学 报 (医 学 版)56卷4期 -秦莹莹,等.卵巢早衰的免疫学病因及免疫干预研究进展 \=-Variable causes and pathogeneses have been identified, including chromosome abnormalities, genetic mutations, autoimmune dysfunctions, and iatrogenic, metabolic or infectious factors, whereas a large proportion of causes still remain unknown. Autoimmune dysfunctions are involved in the pathogenesis of 10%-30% of POF cases, evidenced by autoimmune oophoritis complicated with other autoimmune disorders. However, the contribution of autoimmune deregulation to the pathogenesis of ovarian follicles depletion and dysfunction remains elusive. Reliable diagnostic or predictive biomarkers for ovarian autoimmunity are still unavailable at present. The efficacy of immunological interventions needs evidence-based medicine and high quality clinical trials to confirm.
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    Research advances in the pathogenesis of endometriosis
    WANG Guoyun, YUAN Ming, JI Miaomiao
    Journal of Shandong University (Health Sciences)    2019, 57 (6): 33-39.   DOI: 10.6040/j.issn.1671-7554.0.2019.521
    Abstract538)      PDF(pc) (1825KB)(397)       Save
    Endometriosis(EMs)is a common gynecological disease with a prevalence of 10% in women of childbearing age. The main symptoms of EMs are pain and infertility, which seriously affect patients quality of life. However, 山 东 大 学 学 报 (医 学 版)57卷6期 -王国云,等.子宫内膜异位症发病机制研究进展 \=- there is no EMs-specific, non-invasive diagnostic method yet. Currently, EMs is treated with surgery or hormonoth-erapy, both of which have side effects and high recurrence rate. The ambiguity of EMs pathogenesis limits the diagnosis and treatment. The mainstream theory is retrograde menstruation, but the latest research shows that EMS is a complex chronic disease, which may be induced by the menstrual blood flow, while the complex interaction between genetic, immune and environmental factors plays a more important role. This paper will review the pathogenesis theories and latest research advances, especially the new research fields of EMs, and propose the problems in the EMs mechanism research.
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    Rapid and accurate assessment of pulmonary hypertension in rats via diaphragmatic puncture method
    ZHAO Zihui, WANG Xuyang, ZHANG Peng, FANG Ningning, SHI Duanbo, YANG Fan, YANG Xiaomei, WU Jianbo
    Journal of Shandong University (Health Sciences)    2024, 62 (2): 20-28.   DOI: 10.6040/j.issn.1671-7554.0.2023.0987
    Abstract70)      PDF(pc) (10548KB)(67)       Save
    Objective To establish a simple, rapid and accurate method to measure the right ventricular pressure in rats. Methods A total of 30 male SD rats aged 8-10 weeks were randomly divided into 3 groups: the trans-thoracic right ventricular puncture manometry group(TTM group, n=10), trans-diaphragmatic puncture manometry group(TDM group, n=10), and the monocrotaline-induced pulmonary hypertension with trans-diaphragmatic manometry group(MCT-TDM group, n=10). The MCT-TDM group underwent subcutaneous injection of monocrotaline through the thoracoabdominal side(60 mg/kg), while the TTM and TDM groups were injected with an equivalent volume of sterile saline. Four weeks after model establishment, the right ventricular systolic pressure(RVSP)of the TDM and MCT-TDM groups was measured using the trans-diaphragmatic puncture method, followed by thoracotomy to verify the accuracy of the puncture site; RVSP of the TTM group was measured using the trans-thoracic right ventricular puncture method. The operation time, success rate, and RVSP were compared between the two manometry methods. The hearts and lung tissues of the rats were extracted, left and right ventricles were separated, and the right ventricular hypertrophy index(RVHI)was calculated. Lastly, the left lung tissue was stained with hematoxylin and eosin(HE)to evaluate the extent of pulmonary microvascular lesions. Results The time taken from anesthesia to successful measurement of RVSP was(6.83±1.36)minutes for the TTM group,(8.13±1.25)minutes for the TDM group, and(8.32±1.23)minutes for the MCT-TDM group. The success rate were 70% for the TTM group, 100% for the TDM group, and 90% for the MCT-TDM group. There were no significant differences in RVSP and RVHI between the TDM and TTM groups(P>0.05). The RVSP and RVHI in the MCT-TDM group were significantly higher than those in the TDM group(P<0.001). In the MCT-TDM group, the pulmonary arterioles showed notable thickening of the vessel walls and narrowing of the lumens. Conclusion Measuring the right ventricular pressure with diaphragmatic puncture technique negates the need for thoracotomy and circumvents the use of costly equipment. It enables precise, swift, and straightforward assessment of the right ventricular hemodynamics, holding considerable value for widespread application.
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    Neurosurgery plays a key role in brain science research
    Jizong ZHAO
    Journal of Shandong University (Health Sciences)    2020, 1 (8): 1-4.   DOI: 10.6040/j.issn.1671-7554.0.2020.617
    Abstract1260)   HTML26)    PDF(pc) (1488KB)(493)       Save

    The 21st century is the century of brain. Neurosurgery has a unique advantage in brain research as it can directly observe patients' brain. This article will elaborate the following domains of neurosurgery, including cognitive function research, docking brain-like intelligence transformation, and innovative combination of medicine and engineering.

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    Contrast quantity and injection velocity in coronary angiography with 64 multi-detector CT

    ZHAO Lei,ZHAO Qiao-jing,WANG Xi-lin,ZHANG Wei,SHAO Guang-rui

    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1056)      PDF(pc) (356KB)(444)       Save

    Objective〓〖WTBZ〗To explore the effect of contrast quantity and injection velocity in 64 multi-detector CT. 〖WTHZ〗Methods〓〖WTBZ〗Ninety patients diagnosed as or suspected coronary heart diseases (CAD) were subjected to CT coronary angiography using 64 multi-detector CT. Based on contrast quantity and injection velocity, they were divided into three groups: group A(65mL, 5.0mL/s), group B (45mL,5.0mL/s) and group C(45mL,4.5mL/s).The imaging was analyzed by the coronary modified fraction method, American Heart Association. 〖WTHZ〗Results〓〖WTBZ〗There were no statistical differences between group A and B and between group A and C. 〖WTHZ〗Conclusion〓〖WTBZ〗Contrast quantity and injection velocity may be decreased, but the imaging should still be clear.

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    Expression of mucins (MUC1, MUC2, MUC5AC and MUC6) and
    their significance in invasive ductal breast carcinoma
    ZHUANG Ting1, XUE Mei2, FANG Ai-ju3, GUAN Bing-xin1, LIANG Yan1, WANG Zhi-hui2, WANG Yan1, MENG Bin1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (5): 107-111.  
    Abstract2571)      PDF(pc) (2964KB)(877)       Save

     Objective   To evaluate expression of several mucins in invasive breast ductal carcinoma and the correlation with clinicopathological features. Methods   Tissue microarray (TMA) and immunohistochemical(IHC) technologies were used to evaluate expression of several mucins (MUC1, MUC2, MUC5AC and MUC6) in 207 cases of invasive breast ductal carcinoma and to analyze their correlation with clinicopathological features. Results   MUC1 expression was detected in 89.4% of cases and its expression was associated with lower histological grade, estrogen receptor (ER) positive and absence of lymph node (LN) metastasis. The subcellular localization of MUC1, but not expression level, had a prognostic value in postoperative recurrence and metastasis. MUC2 expression was noticed only in 4.3% of all cases. MUC5AC and MUC6 were expressed in 42.0% and 22.2% of cases respectively. There were associations between MUC5AC and lower histological grade as well as ER-positive, and MUC6 was positively correlated with HER-2. But there was no association between mucins and other clinicopathological variables. Conclusion   WhileMUC1 is expressed in the majority of the cases, others (MUC2, MUC5AC, MUC6) are not. Ectopic expression of MUC1 might be a potential prognostic indicator, which needs further study for confirmation.

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    Current status and prospect of viral vaccines research and development
    Kai WANG
    Journal of Shandong University (Health Sciences)    2021, 59 (5): 8-14.   DOI: 10.6040/j.issn.1671-7554.0.2021.0273
    Abstract3014)   HTML173)    PDF(pc) (1285KB)(1231)       Save

    Virus infection can cause several diseases in human. In the past decades, outbreaks and epidemics of respiratory infectious diseases caused by coronary viruses, especially coronavirus disease 2019 (COVID-19), have become serious health hazards and posed huge challenges to public health. The development and application of viral vaccines have been an important means to counteract the spread of viruses. Historically, poliovirus and hepatitis B virus vaccines have successfully suppressed the transmission of diseases and provided a model for the development of vaccines. Recently, the emergence of novel technologies and platforms such as recombinant viral vector and mRNA vaccine has provided new approaches to the development of vaccines against emerging infectious diseases including COVID-19. This paper reviews the current situation and progress of platforms, summarizes problems to be solved, and forecasts the directions of viral vaccines development.

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    New practice of radio-immunotherapy for lung cancer
    YU Jinming, YAN Weiwei, CHEN Dawei
    Journal of Shandong University (Health Sciences)    2021, 59 (9): 1-8.   DOI: 10.6040/j.issn.1671-7554.0.2021.0949
    Abstract1038)   HTML25)    PDF(pc) (1451KB)(319)       Save
    In recent years, immunotherapy combined with radiotherapy(iRT)has attracted great attention in the field of oncology, and hundreds of large clinical trials on iRT are currently underway. Radiotherapy can cause immunogenic death of tumor cells, promote the release and presentation of tumor antigens, and activate T cells. Radiotherapy can also reshape the tumor microenvironment from immunosuppression to immune activation. Immune checkpoint inhibitors can not only relieve T cell inhibition but also normalize tumor vessels so as to improve tumor hypoxia and increase radiosensitivity, which is the basic principle of synergistic anti-tumor effect of radiotherapy and immunity. Now iRT is becoming more and more mature, but it also faces many challenges. This paper will review the development of immunotherapy and radiotherapy in recent years, and explore the timing, fractional dose and treatment-related toxicity of iRT.
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    Research progress of drug resistance in ovarian cancer and targeted therapy strategy
    HA Chunfang, LI Ruyue
    Journal of Shandong University (Health Sciences)    2021, 59 (9): 117-123.   DOI: 10.6040/j.issn.1671-7554.0.2021.0956
    Abstract1321)   HTML13)    PDF(pc) (1361KB)(532)       Save
    Cytoreductive surgery combined with chemotherapy is the major treatment of Epithelial ovarian cancer(EOC). The application of no residual disease(R0)targeted ovarian cancer debulking with individualized chemotherapy has greatly improved the five-year survival rates. However, the drug resistance and recurrence of advanced patients are still a difficult problem for clinicians and researchers in this field. Chemotherapy resistance is a hot and difficult spot to be solved urgently due to unclear etiology and complex mechanism. In recent years, the basic and clinical researches on the mechanism of drug resistance in ovarian cancer mainly include drug transport, drug metabolism, apoptosis, DNA damage repair and epigenetic changes. And some studies have shown that autophagy, altered metabolism, tumor microenvironment and tumor stem cells also have played an important role in drug resistance in ovarian cancer. The strategy targeting these mechanisms has provided a new method to increase sensitivity to chemotherapy and improve the prognosis.
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    Dilemmas and thoughts on solutions of adherence to chronic disease management
    WANG Kefang, WU Chen
    Journal of Shandong University (Health Sciences)    2021, 59 (9): 124-129.   DOI: 10.6040/j.issn.1671-7554.0.2021.0911
    Abstract1196)   HTML18)    PDF(pc) (2692KB)(411)       Save
    Chronic diseases are a major public concern resulting in substantial social and economic consequences globally. The suboptimal adherence to strategies of chronic disease management is the “bottleneck” issue that should be addressed across countries. This paper describes the nested theoretical model integrating the process of chronic disease management and adherence to management strategies derived from both empirical and theoretical evidence, summarizes the arguments on the “salami slicing” actions adopted by researchers in adherence promotion studies, and proposes our thoughts on solutions of adherence to management strategies via the lens of ontology, epistemology, and methodology. Efforts in this paper may imply future researchers to develop a system to promote and maintain adherence to management strategies among patients with chronic diseases, and facilitate the chronic disease management in China.
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    Research progress of animal models of adenomyosis
    WANG Guoyun, PAN Zangyu
    Journal of Shandong University (Health Sciences)    2022, 60 (7): 48-55.   DOI: 10.6040/j.issn.1671-7554.0.2022.0263
    Abstract778)   HTML9)    PDF(pc) (1206KB)(388)       Save
    Adenomyosis refers to the pathological changes caused by the invasion of endometrium (including glands and stroma) into the myometrium. The main clinical symptoms include menorrhagia, severe dysmenorrhea and infertility, which have a serious impact on the physical and mental health of patients. Adenomyosis often occurs in women of childbearing age, but its etiology remains unclear. There is still no specific diagnostic method, and the treatment methods are limited. Researches on the pathogenesis, characteristic diagnosis and targeted treatment are urgently needed. The establishment and application of animal models are significant to solve the above problems since many studies have shown that adenomyosis is not unique to humans. The alternative experimental animals have reproductive system similar to that of human women, which is conducive to the study of mechanism. This paper reviews the establishment and application of animal model of adenomyosis.
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    Tumescent technique in multi-position liposuction
    CAI Jing-long,ZHU Gui-ying,DONG Hong,JIANG Du-yin,XU Bin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1231)      PDF(pc) (344KB)(1006)       Save
    Objective: To study the clinical effect of tumescent technique in multi-position liposuction. Methods: Tumescent liposuction technique was used on 1200 different sites of 500 cases. The pre- and post-operative treatment, the tumescent liquid amount, the usage of microcannula in the superficial fat layer, the lidocaine dosage, instruments and operation methods, and the maintenance of body fluid equilibrium were all well controlled. Results: There was good efficiency such as fewer complications, obviously shrinking skins and smooth operation areas. Conclusion: Tumescent technique can meet the need of multi-position and large-volume liposuction. It is a safe and effective body sculpture technique.
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    Microsurgical treatment for craniopharyngioma: analysis of 59 cases
    ZHANG Lei, LI Xingang, LI Gang, XU Shujun, WANG Donghai, WANG Xinyu, ZHAO Peng, LI Xueen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (7): 122-125.  
    Abstract1002)      PDF(pc) (477KB)(658)       Save

    Objective    To explore the operative approach in microsurgical treatment of craniopharyngioma and to evaluate its effects. Methods   Clinical manifestations, imaging examinations, surgical approaches, tumor resection extent and follow-up data of 59 cases of craniopharyngioma were retrospectively analyzed. Results    43 cases of tumors were totally removed, and 16 subtotally removed. No patients died. After 5 months to 3 years of follow-up, 3 cases of recurrence were found. The symptoms and signs in the 50 patients were improved in different degrees. Conclusion    The interhemispheric approach is more safe and effective for removing craniopharyngioma.

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    Changes in mandibular direction during and after protraction headgear treatment
    WANG Na-na, CAO Cong, LIU Dong-xu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2013, 51 (9): 95-99.  
    Abstract714)      PDF(pc) (1198KB)(476)       Save

    Objective     To observe and evaluate the mandibular changes in growing Class Ⅲ malocclusion patients treated with protraction headgear. Methods    This study consisted of 30 adolescent Class Ⅲ malocclusion subjects treated with protraction headgear and 30 untreated controls from the Department of Orthodontic, School of Stomatology, Shandong University. All the subjects had their lateral cephlometrics taken before and after the treatment or observation period.The cephalometric data including variables assessing sagittal and vertical relationships of maxillary was analyzed and the direction of mandibular displacement was evaluated according to a structural superimposition method. Results   The results showed significant improvements in dentofacial profile after 6 months of maxillary protraction. During the treatment or observation period, the mean mandibular rotation between the treatment and control groups had significant difference (P<0.05). It was clockwise rotation for the treatment group and anticlockwise rotation for the control group. The mean rotation in the treatment and control groups were 0.8° and -0.6°, respectively. A more horizontal movement of the mandibular could be observed in the control group during the study period. Conclusion    The mandibular of the treatment group produces clockwise rotation while the control group has a trend of anticlockwise rotation after protraction headgear treatment.

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    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2014, 52 (Z1): 206-206.  
    Abstract1238)      PDF(pc) (2649KB)(293)       Save
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    Progress on the activation of endogenous stem cells and functional repair in LIPUS mechanotransduction
    XIA Shujie, GUAN Yanjie
    Journal of Shandong University (Health Sciences)    2021, 59 (9): 37-42.   DOI: 10.6040/j.issn.1671-7554.0.2021.0966
    Abstract1120)   HTML4)    PDF(pc) (1522KB)(477)       Save
    In recent years, mechanical therapies represented by low-intensity pulsed ultrasound(LIPUS)have been widely used in rehabilitation medicine, fracture repair, nerve injury repair, urinary system diseases and other fields. Current studies have shown that LIPUS may participate in tissue repair by activating endogenous stem cells, but the specific mechanism by which mechanical force activates stem cells through the transformation of the biological chain is still unclear. Therefore, further exploration of the conduction process of mechanotransduction will help to reveal the mechanism of LIPUS and provide reference for subsequent researches and clinical applications.
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    Medico-engineering cooperation: a strategic plateau of neurosurgery innovation and development in the era of precision medicine
    YANG Jun
    Journal of Shandong University (Health Sciences)    2021, 59 (9): 83-88.   DOI: 10.6040/j.issn.1671-7554.0.2021.0967
    Abstract1436)   HTML11)    PDF(pc) (1413KB)(399)       Save
    Since the concept of “precision medicine” was formally put forward in 2015, the accurate diagnosis and treatment of diseases has become the main theme of clinical medicine. Neurosurgery is a specialty with relatively late development, strong specialty, high difficulty in practice, long training period and steep learning curve in the major surgical system. Due to the continuous maturity, popularization, normalization and standardization of medico-engineering cooperation, neurosurgery has achieved rapid development. In the current era of precision medicine, neurosurgery has entered a critical development period, and medico-engineering cooperation will become an important strategic plateau for the innovation and development of the discipline. Herein, we will focus on the breakthroughs of medico-engineering cooperation in the field of neurosurgery, the history and prospects of neurosurgery innovation.
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    A mendelian randomization analysis on the causal associations between amino acids and type 2 diabetes
    ZHANG Tianxin, ZHANG Ting, HUANG Xin, HAN Jiayi, WANG Shukang
    Journal of Shandong University (Health Sciences)    2023, 61 (5): 102-107.   DOI: 10.6040/j.issn.1671-7554.0.2023.0085
    Abstract567)      PDF(pc) (955KB)(166)       Save
    Objective To investigate the bidirectional causal association between amino acids and type 2 diabetes(T2D). Methods Data of genome-wide association study on amino acids and T2D were collected. Inverse variance weighted method, weighted median method, MR-Egger regression method, MR robust adjusted profile score method and MR pleiotropy residual sum and outlier method(MR-PRESSO)were adopted for two-sample bidirectional mendelian randomization(MR)analysis. Cochrans Q test, MR-Egger intercept test, and MR-PRESSO global test were used to evaluate the heterogeneity and pleiotropy of the results. Results The results of inverse variance weighted method showed that leucine(OR=1.239, 95%CI: 1.078-1.424, P=0.002 5)and valine(OR=1.222, 95%CI: 1.071-1.394, P=0.002 9)increased the risk of T2D, while glycine(OR=0.885, 95%CI: 0.831-0.942, P=0.000 1)decreased the risk. The results of reverse MR analysis showed that the risk of T2D was causally associated with increased leucine, isoleucine, valine, alanine, phenylalanine and tyrosine, and decreased glycine. The results of multiple MR methods suggested robustness of causal associations and no potential pleiotropy was found in the sensitivity analysis. Conclusion Higher levels of leucine and valine and lower level of glycine have bidirectional causal associations with T2D risk. T2D has positive causal effects on isoleucine, alanine, phenylalanine and tyrosine.
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    Surgical treatment of Stanford type A aortic dissection
    SUN Yong-hui, ZOU Cheng-wei, LI De-cai, LI Hong-xin, WANG Zheng-jun, ZI Jie, ZHANG Wen-long, ZHANG Hai-zhou, FAN Quan-xin, WANG An-biao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2011, 49 (1): 71-.  
    Abstract1214)      PDF(pc) (414KB)(712)       Save

    Objective    To review surgical treatment of type A aortic dissection in 65 patients and to investigate the effectiveness of surgical approaches. Methods    Between September, 2003 and April, 2010, 65 patients,  including 45 males and 20 females from 26 to 58 (mean 41.6 ± 9.5) years old, with Stanford type A aortic dissection underwent surgical treatment. Different surgical approaches were adopted according to the rupture site, extent of dissection and aortic valvular insufficiency, including ascending aorta replacement only (10 cases), ascending aorta replacement + arch or semi-arch replacement (13 cases), ascending aorta replacement + arch or semi-arch replacement with stented elephant trunk (27 cases), ascending aorta replacement + four branches covered stent graft implantation (7 cases) and Bentall operation only (8 cases). Concomitant procedures were coronary artery bypass grafting (4 cases), aortic valvuloplasty(12 cases), aortic valve replacement(8 cases ) or Bentall operation (6 cases). Deep hypothermic circulation arrest with selective cerebral perfusion was employed in cases of aortic arch replacement and/or  endovascular stent repair. Results    In the operations, the CPB, clamp and circulation arrest time were 86 -350 (188.3±60.9), 60-248 (101.1±40.0)  and 20-120 (53.9±24.3) minutes, respectively. There were two deaths  early  after operation. Onedied from low cardiac output syndrome  and the other died from renal failure. Postoperative intensive care stay was 1~63 (4.2±8.8) days and the hospital stay was 13~106 (24.8±13.8) days. Complications  consisted of motor aphasia(n=1), cerebral embolism(n=1), pulmonary infection(n=3), renal failure(n=1), hemorrhage(n=5), mental disorder(n=4), tracheotomy (n=1), and voice hoarseness(n=1). Conclusion     Aortic dissection is the most common catastrophic event that involves the aorta, however, the prospects of early surgical treatment are satisfying.  The key points are quick, correct diagnosis and proper surgical approaches.

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    Associations of maternal methionine synthase A2756G and methionine synthase reductase A66G polymorphisms with neural tube defects of the offspring: A meta-analysis
    ZHANG Bing-zhen, DAI Bing-qin, ZHANG Xing-liang, WANG Zhi-ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (7): 125-132.  
    Abstract1089)      PDF(pc) (3609KB)(932)       Save

    Objective   To evaluate associations of maternal methionine synthase (MTR) A2756G and methionine synthase reductase(MTRR) A66G polymorphisms with the offspring′s risk for neural tube defects (NTDs). Methods   The retrieval strategy and criteria for inclusion and exclusion were made. Literature was identified by searches for case-control studies on maternal MTR A2756G or MTRR G66A polymorphism and risk of NTDs in the offspring published in English or Chinese from following databases: China Biology Medical Literature Database(CBM), Database of Chinese Scientific and Technical Periodicals(VIP), China National Knowledge Infrastructure(CNKI), Digital Journal Full-text Database(CHINAINFO), PubMed and Web of Science from January 1990 to October 2010.Combined OR values and 95% CI were calculated with Review Manager 5.0. Results   18 eligible studies were included in the metaanalysis, among which 11 studies(907 cases and 1,978 controls) on maternal MTR A2756G polymorphism, and 11 (1123 casesand 1700 controls) on maternal MTRR A66G polymorphism. Statistical analysis of the combined data showed that there was no significant association between maternal MTR A2756G polymorphism and the risk of NTDs, while there were significant associations between maternal MTRR A66G polymorphism and the risk of NTDs in GG/AG vs. AA, GG vs. AA, AG vs. AA, GG vs. AG/AA and G vs. A genetic models, and their pooled OR values and 95% CI were 1.89(1.282.78), 1.68(1.312.16), 1.77(1.18-2.66), 1.28(1.06-1.55) and 1.35(1.12-1.63), respectively. Conclusion   Maternal MTRR A66G polymorphism is a risk factor for NTDs.

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    Antimicrobial effect of diallyl trisulfide on Helicobacter pylori biofilms
    LIU Wei, YU Han, LI Yu-tang, LIU Xu-jing, LI Wen, SHAN Yu-qun, SUN Yun-dong, ZHOU Ya-bin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2013, 51 (10): 49-53.  
    Abstract826)      PDF(pc) (2037KB)(499)       Save

    ObjectiveTo investigate the antimicrobial effects of diallyl trisulfide (DATS) on Helicobacter pylori(H.pylori) biofilms and to explore the drug-resistance mechanism of biofilms. Methods  The minimal inhibitory concentration of selected antimicrobials were measured by broth microdilution assay; The biofilm models in vitro were established on agar plate; The cell activity of planktonic and sessile H.pylori treated with selected antimicrobials was measured with XTT reduction assay; The morphology and activity changes of H.pylori in deep biofilm were observed by confocal laser scanning microscopy. Results  Both clarithromycin and DATS elicited strong antimicrobial activity against planktonic and sessile H.pylori; Different concentrations of clarithromycin significantly reduced the cell activity of H.pylori biofilm; The cell activity of the DATS treated group was significantly lower than that of the control group and the clarithromycin treated group. It had statistical differences. Conclusion  DATS elicits strong antimicrobial activity against both planktonic and sessile H.pylori. It may act as an alternative chemotherapeutic agent for multidrug-resistant bacterial strains.

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    Correlation of QuantiFERON-TB gold in-tube test result with age of adults
    BU Fanfeng, SUN Xiaxia, LIANG Xiaoling
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2016, 54 (6): 87-90.   DOI: 10.6040/j.issn.1671-7554.0.2015.493
    Abstract1649)      PDF(pc) (1530KB)(594)       Save
    Objective To explore the relationship between age and release of interferon gamma in blood of adults through the correlation analysis of age and QuantiFERON-TB gold in-tube test(QFT-GIT)results. Methods A total of 168 patients with positive results of QFT-GIT were divided into three groups according to the age:18 to 40 years group, 41 to 65 years group and elder than 65 years group. The changes of IFN-γ response to TB-specific antigen(TB Ag-Nil)and non-specific phytohemagglutinin(Mitogen-Nil)were compared among the three groups. Spearman correlation analysis was used to calculate the correlation coefficient value γ. Linear regression analysis was used to show the change. Results The change trend of TB Ag-Nil and Mitogen-Nil in 18 to 40 years group and 41 to 65 years group were not statistically significant(P>0.05). However in patients elder than 65 years, the levels of TB Ag-Nil and Mitogen-Nil decreased significantly with age increasing(P<0.05). Conclusion For patients of 18 to 65 years, the results of QFT-GIT are not related to the age, but for those elder than 65 years, TB Ag-Nil and Mitogen-Nil is negatively related to the age.
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    A new field of therapeutic interventional pulmonology——clinical application of tracheal T tube
    HUANG Haidong, ZHENG Guiliang, WANG Qin, YANG Yuguang, LI Qiang, BAI Chong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2017, 55 (4): 23-29.   DOI: 10.6040/j.issn.1671-7554.0.2017.165
    Abstract1444)      PDF(pc) (1591KB)(613)       Save
    With the rapid development of rigid bronchoscopy and interventional pulmonology in China, Chinese pulmonologists who are engaged in the practice of interventional pulmonology now have the conditions of tracheal T tube placement by rigid bronchoscope technique for the treatment of proximal tracheal stenosis. The tracheal T tube placement by rigid bronchoscope technique has a certain minimally invasive advantage compared to that by traditional surgery technique. However, all the clinical problems usually can not be solved by a single technique due to the complex situations of laryngotracheal stenosis. Better clinical results and low complications for laryngotracheal stenosis are related to the understanding of the difference between two techniques, developing multidisciplinary treatment approaches and strictly controlling of the indications of tracheal T tube placement.
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    Infertility stress and posttraumatic stress disorder in infertile women: mindfulness as a mediator and moderator
    BAI Lu, ZHANG Yuqing, FANG Yuying
    Journal of Shandong University (Health Sciences)    2021, 59 (2): 83-87.   DOI: 10.6040/j.issn.1671-7554.0.2020.1516
    Abstract927)      PDF(pc) (1077KB)(317)       Save
    Objective To investigate the influencing factors of posttraumatic stress disorder(PTSD)in infertile women and to evaluate the protective effect of mindfulness on the relationship between infertility stress and PTSD. Methods Totally 362 patients in a reproductive center in Shandong Province were enrolled and surveyed with Fertility Problem Inventory(FPI), Mindful Attention Awareness Scale(MAAS), PTSD Cheeklist-Civilian Version. The mediating effect and moderating effect of mindfulness were analyzed with hierarchical regression analysis. Results The average score of PTSD of infertile women was 31.27±11.68, and 24.2% of patients had score ≥38. Logistic regression analysis showed that mindfulness partially mediated the effect of infertility stress on PTSD, with a mediating effect of 0.188, accounting for 33.04% of the total effect. Mindfulness also moderated the association between infertility stress and PTSD. Conclusion Infertility stress has both direct and indirect effects via mindfulness on PTSD. Mindfulness buffers the effect of infertility stress on PTSD and is a protective factor of PTSD in infertile women.
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    Knockdowa of LncRNA H19 inhibits sympathetic nerve remodeling after myocardial infarction by regulating nerve growth factor
    ZHANG Xueli, ZHENG Lu, WANG Yu, WANG Kang, YAN Suhua
    Journal of Shandong University (Health Sciences)    2021, 59 (5): 73-81.   DOI: 10.6040/j.issn.1671-7554.0.2020.1534
    Abstract789)      PDF(pc) (9043KB)(197)    PDF(mobile) (8538KB)(4)    Save
    Objective To explore the roles of H19 and nerve growth factor(NGF)in the development of ventricular arrhythmias(VAs)after myocardial infarction(MI). Methods MI rat models were established by left coronary artery occlusion. (1)A total of 14 healthy Sprague Dawley(SD)rats were randomly divided into the MI group and sham group, with 7 rats in each group. Seven days after MI was induced, the rats were sacrificed to determine the H19 expression with qRT-PCR. (2)A total of 32 rats were randomly divided into 4 groups: sham+NC group, sham+siH19 group, MI+NC group and MI+siH19 group, with 8 rats in each group. H19-siRNA or the control virus was injected into the left ventricular myocardium of the rats. Programmed electrical stimulation was performed 7 days after MI was induced to observe the susceptibility to VAs. The effects of H19 on NGF, tyrosine hydroxylase(TH)and growth-associated protein 43(GAP43)were detected with qRT-PCR, Western blotting and immunofluorescence staining. (3)A total of 24 rats were randomly divided into the MI+NC group, MI+siH19 group and MI+siH19+LV-NGF group for rescue test, with 8 rats in each group. The expressions of NGF, TH and GAP43 were detected with Western blotting and immunofluorescence staining. Results The expression of H19 were up-regulated 7 days after MI was induced(P<0.001). H19 knockdown suppressed the positive staining area of TH and GAP43(P<0.001), decreased the susceptibility to VAs(P<0.01), and inhibited the expression of NGF(Z=-2.402, P=0.016). The inhibitory effect of H19 knockdown on sympathetic remodeling could be saved by overexpressed NGF(PTH<0.001, PGAP43=0.001). Conclusion H19 knockdown after myocardial infarction can inhibit sympathetic nerve remodeling by regulating the expressiou of NGF, thus reducing the incidence of arrhythmia.
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    Advances on the etiology and pathogenesis of osteoporosis
    LUO Xianghang, ZHOU Ruoyu
    Journal of Shandong University (Health Sciences)    2021, 59 (6): 10-14.   DOI: 10.6040/j.issn.1671-7554.0.2021.0324
    Abstract3299)   HTML233)    PDF(pc) (762KB)(943)       Save
    Osteoporosis is the most common metabolic bone disease. The incidence of osteoporosis as well as osteoporotic fracture increases gradually with age, seriously endangering patients health. The core pathogenesis of osteoporosis is that various factors act on some stages of bone remodeling, resulting in stronger bone resorption than bone formation. These factors include genetic factors, age, endocrine and lifestyle. It is of great significance to study the pathogenesis and etiology of osteoporosis for its prevention and treatment.
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    Immunomodulatory effects of bile acid in hepatointestinal diseases
    ZHANG Zhaoying, MA Chunhong
    Journal of Shandong University (Health Sciences)    2021, 59 (9): 30-36.   DOI: 10.6040/j.issn.1671-7554.0.2021.0924
    Abstract787)   HTML11)    PDF(pc) (3885KB)(275)       Save
    Bile acid, the final product of cholesterol catabolism in liver and the main component of bile, is critical for the emulsification and absorption of lipids. Dysregulation of bile acid metabolism is closely related to various hepatic and intestinal diseases, including hepatocellular carcinoma and colorectal cancer. At present, the clinical drugs targeting bile acid are widely utilized. Immune cells play an important role in the process of hepatointestinal diseases. Bile acid is closely related to liver and intestine immune homeostasis, affecting the differentiation and function of various immune cells, and thus affecting the occurrence and development of diseases. In this paper, we will review the regulation effect of bile acid on hepatointestinal immune cells in order to develop new therapeutic methods for hepatointestinal diseases by using bile acid.
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    Status and progress of female fertility protection
    WU Ruifang, LI Changzhong
    Journal of Shandong University (Health Sciences)    2022, 60 (9): 1-7.   DOI: 10.6040/j.issn.1671-7554.0.2022.0916
    Abstract1138)   HTML23)    PDF(pc) (1344KB)(314)       Save
    Fertility protection, as a new and rapidly developing interdisciplinary field in recent years, has made great progress and applied fertility preservative technology(FPT)which plays important roles in preserving fertility and solving infertility problems. FPT such as in vitro cryopreservation of the eggs or ovarian tissues from young tumor patients can protect patients' fertility from disease-related damages or environmental-impacted infertility. Surgical measures with primary purpose to keep fertility can solve problems resulting in infertility and repair or reconstruct the structure and function of the reproductive organs. Assisted reproductive treatment as a complement measure can rate up the success of assisted reproduction. In this article, we will highlight the significant developments of fertility preservation, identify the challenges to be faced and put forward recommendations for the development of fertility preservative technology in China.
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    Ethical issues and considerations in fertility preservation
    HAO Guimin, LUO Zhuoye, WANG Yizhuo
    Journal of Shandong University (Health Sciences)    2022, 60 (9): 47-52.   DOI: 10.6040/j.issn.1671-7554.0.2022.0909
    Abstract810)   HTML9)    PDF(pc) (1282KB)(161)       Save
    As an emerging technology, fertility preservation technology has gradually developed from experimental to clinical application in recent years. On the one hand, it meets peoples growing demand for maintaining fertility. On the other hand, ethical issues inevitably arise when implementing this technology. On the basis of adherence to the ethical principles of respect, beneficence and justice in clinical practice, developing technology, optimizing the allocation of medical resources, improving policies, and strengthening ethical scrutiny may provide methods for solving ethical problems.
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    Impacts of tropical cyclone on human health: latest research progress
    MA Wei, ZHANG Anran
    Journal of Shandong University (Health Sciences)    2018, 56 (8): 29-36.   DOI: 10.6040/j.issn.1671-7554.0.2018.519
    Abstract496)      PDF(pc) (1311KB)(253)       Save
    Tropical cyclone, a natural disaster which may cause huge damages to human beings, has direct and indirect impacts on human health in various ways. It can cause direct casualties through gales, heavy rains and storm tides, and can also indirectly increase mortality and disability rate, incidence of infectious and chronic diseases, through house and health facility damage, movement of population, water pollution, and grain yield reduction. In addition, it can cause psychological and mental damage. Adaptive measures need to be taken both before and after landing of tropical cyclone in order to reduce its impacts on human health.
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    Hazards of common complications in assisted reproductive technology
    HAO Guimin, LUO Zhuoye, CUI Na
    Journal of Shandong University (Health Sciences)    2019, 57 (10): 7-12.   DOI: 10.6040/j.issn.1671-7554.0.2019.724
    Abstract561)      PDF(pc) (1338KB)(169)       Save
    With the rapid development of assisted reproductive technology(ART), the risks and challenges accompanied cannot be ignored. Ovarian hyperstimulation syndrome, adnexal torsion, hemorrhage after oocyte retrieval, infection, organ damage, multiple pregnancy, and ectopic pregnancy are the common complications of ART. These complications may adversely affect pregnancy outcomes, having an impact on health-related quality of life or even being life-threatened. Serious concern is warranted in terms of risk factors and safety management of ART. Establishing standardized quality management system to identify risk factors in advance, taking effective prevention measures, identifying anomalies in time and providing individualized treatment will make ART more satisfactory.
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