JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Effects of caspase-3 activation and cytochrome c release in cerebral ischemia reperfusion injury

ZHANG Wen-hua, ZHOU Lu, XU Shu-jun, CHENG Teng, JIA De-ze, ZHOU Mao-de, WU Cheng-yuan   

  1. Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2007-06-18 Revised:1900-01-01 Online:2007-12-24 Published:2007-12-24
  • Contact: ZHANG Wen-hua

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Abstract: ObjectiveTo evaluate the effects of caspase-3 activation and cytochrome c release in cerebral ischemia reperfusion induced neuronal cell death. MethodsFocal cerebral ischemia C57L mice model was established by the middle cerebral occlusion (MACo) method. Caspase-3 activity was determined by an enzyme activity assay at different time points after ischemia reperfusion. Caspase-3 activation and cytochrome c release were determined by immunohistology staining and Western blot. Results①Caspase-3 activation immediately occurred at 3h after ischemia reperfusion and was gradually increased till to the peak at 12h to 24h. ②Caspase-3 was significantly activated at 6h after completion of ischemia reperfusion and it was increased by 7.6 times in comparison with the sham control (P＜0.01). ③Cytochrome c release occurred at 6h after completion of ischemia reperfusion and it was increased by 8.5 times in comparison with the sham control (P＜0.01). Conclusions Caspase-3 activation and cytochrome c release play a critical role in neuronal cell death induced by cerebral ischemeia reperfusion. It provides insights into the mechanism of events involved in ischmeia reperfusion injury.

Key words: Cerebral ischemia, Reperfusion injury, Caspase-3, Cytochrome c

CLC Number: 

  • R743.33
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