Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (5): 11-19.doi: 10.6040/j.issn.1671-7554.0.2022.1081

• 基础医学 • Previous Articles    

IFN-γ promots the proliferation of NHL by up-regulating PD-L1 via PI3K/Akt/mTOR pathway

XIE Xiaoli1,2, QIU Yu1,2, WANG Lijuan1,2   

  1. 1. Central Laboratory, Linyi Peoples Hospital, Linyi 276000, Shandong, China;
    2. Linyi Key Laboratory of Tumor Biology, Linyi 276000, Shandong, China
  • Published:2023-05-15

Abstract: Objective To explore the effects of interferon-γ(IFN-γ)on the proliferation of non-Hodgkin lymphoma(NHL)by regulating the expression of programmed cell death ligand 1(PD-L1)and the mechanism. Methods The expression of PD-L1 in various NHL cell lines was detected with flow cytometry. After NHL cell lines U937 and Jeko-1 were treated with IFN-γ, the expression of PD-L1 was detected with flow cytometry and quantitative real-time PCR(qRT-PCR). After NHL cells were co-cultured with INF-γ and phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR)signaling pathway inhibitor, change in the expression of PD-L1 was detected with Western blotting and flow cytometry. Cell proliferation was detected with cytotoxicity detection kit(CCK-8)and carboxyfluorescein diacetate succinimidyl ester(CFSE)staining incorporation method. Cell proliferation rate was detected with Edu labeling method. Cell cycle was detected with flow cytometry. Results The qRT-PCR results showed that PD-L1 was expressed on the surface of various NHL cell lines. After IFN-γ treatment, the mRNA and protein expressions of PD-L1 in U937 and Jeko-1 cells were increased. After U937 and Jeko-1 cells were co-treated with IFN-γ and signaling pathway inhibitors, the PI3K/Akt/mTOR signaling pathway inhibitor inhibited the up-regulation of PD-L1 expression by IFN-γ, and cell proliferation was inhibited. Conclusion IFN-γ can up-regulate the expression of PD-L1 in NHL cells through the PI3K/Akt/mTOR signaling pathway; IFN-γ promotes NHL cell proliferation by up-regulating the expression of PD-L1.

Key words: Non-Hodgkin lymphoma, Programmed cell death ligand 1, Interferon-γ, Phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin

CLC Number: 

  • R574
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