MMP-9/TIMP-1在大鼠弥漫性脑损伤后早期的表达及意义

山东大学学报(医学版)

MMP-9/TIMP-1在大鼠弥漫性脑损伤后早期的表达及意义

宋林杰¹，张秀萍²，王盽¹，董亚南¹，姜勇¹，赵洪洋³，朱贤立³

1. 山东大学附属济南市中心医院神经外科，济南 250013；
2. 山东大学附属济南市中心医院医学实验诊断中心，济南 250013；
3. 华中科技大学同济医学院附属协和医院神经外科，武汉 430022

收稿日期:2008-07-09 修回日期:1900-01-01 出版日期:2008-11-16 发布日期:2008-11-16
通讯作者: 宋林杰

Expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 after diffuse brain injury and their significance in rats

SONG Lin-jie¹, ZHANG Xiu-ping², WANG Chong¹, DONG Ya-nan¹, JIANG Yong¹, ZHAO Hong-yang³, ZHU Xian-li³

1. Deparment of Neurosurgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China;
2. Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China;
3. Deparment of Neurosurgery, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China

Received:2008-07-09 Revised:1900-01-01 Online:2008-11-16 Published:2008-11-16
Contact: SONG Lin-jie

摘要/Abstract

摘要： 目的探讨基质金属蛋白酶-9及其抑制剂TIMP-1在大鼠弥漫性脑损伤后早期的表达变化与继发脑损伤的关系。方法荧光实时定量RT-PCR分别测定MMP-9mRNA和TIMP-1mRNA在大鼠弥漫性脑损伤早期不同时程的表达，干湿重法测定脑水分含量，光镜和电镜观察血脑屏障结构的变化。结果与假手术对照组相比，外伤后1?h大鼠脑组织中MMP-9mRNA开始增加，伤后12h达到高峰并持续7?d（P<0.01）；TIMP-1mRNA表达6h明显升高，24h达高峰，表达量增加1倍，并持续7d（P<0.01）。脑组织含水量比假手术对照组明显增加，光镜及电镜下可见炎性反应和毛细血管的超微结构破坏、神经元变性水肿和坏死，72h损伤最重。结论大鼠外伤后诱导MMP-9mRNA和TIMP-1mRNA表达增加，可能参与了弥漫性脑损伤后早期脑水肿和神经元损伤的继发脑损害病理过程。

关键词: 大鼠, Sprague-Dawley, 金属蛋白酶1组织抑制剂, 血脑屏障, 明胶酶B, 脑损伤

Abstract: To explore whether expressions of matrix metalloproteinase-9 and tissuce inhibitor of metalloproteinase-1 in early diffuse brain injured tissues have a correlation with second brain injury in rats. Methods Real-Time quantitative PCR was used to determine expressions of MMP-9mRNA and TIMP-1mRNA, the dry-wet method to determine the containing water measure in the brain, and histological techniques and electron microscopy to determine the ultrastructural blood brain barrier at different time-points after diffuse brain injury in rats. Results Compared with the sham-operated group, expression of MMP-9mRNA started to increase at the first hour, peaked at the 12th hour(P<0.01) and persisted up to one week. Expression of TIMP-1mRNA also peaked at the 24th hour and persisted up to one week (P<0.01). Ultrastructures of the neurons were injured, edema and necrosis were diffuse and distinct at the 72 th hour under light microscopy and electron microscopy. ConclusionsExpressions of MMP-9mRNA and TIMP-1mRNA are increased after diffuse brain injury in rats, indicating that MMP-9 and TIMP-1 are closely related to brain edema and neuronal damage in early second brain injury in rats.

Key words: Brain injury, Gelatinasc B, Tissue inhibitor of metalloproteinase1, Blood brain barrier, Rats, sprague-dawley

中图分类号:

R651.1

宋林杰,张秀萍,王盽,董亚南,姜勇,赵洪洋,朱贤立 . MMP-9/TIMP-1在大鼠弥漫性脑损伤后早期的表达及意义[J]. 山东大学学报(医学版), 2008, 46(11): 1041-1044.

SONG Lin-jie,ZHANG Xiu-ping,WANG Chong,DONG Ya-nan,JIANG Yong,ZHAO Hong-yang,ZHU Xian-li . Expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 after diffuse brain injury and their significance in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(11): 1041-1044.

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