地钱愈伤组织的诱导及其细胞培养条件的建立

山东大学学报(医学版)

地钱愈伤组织的诱导及其细胞培养条件的建立

尹德明，温学森，娄红祥

山东大学药学院天然药物化学教研室，济南 250012

收稿日期:2007-11-06 修回日期:1900-01-01 出版日期:2008-04-16 发布日期:2008-04-16
通讯作者: 娄红祥

Initiation of callus tissues and establishment of suspension culture of Marchantia polymorpha L. cells

YIN De-ming, WEN Xue-sen, LOU Hong-xiang

Department of Natural Drug and Chemistry, School of Pharmaceutical Science, Shandong University, Jinan 250012, China

Received:2007-11-06 Revised:1900-01-01 Online:2008-04-16 Published:2008-04-16
Contact: LOU Hong-xiang

摘要/Abstract

摘要： 目的探计对地钱愈伤组织的诱导和悬浮细胞培养的条件。方法用MSK2和MS培养基对地钱配子体进行愈伤组织诱导，运用模糊评判对MSK-2培养基生长激素组合进行综合性状评价。结果MSK-2和Ms（2mg/L 6BA）均能诱导出地钱愈伤组织，其中MSK2的诱导效果较好。光照（3000～8000 lux）能有效地促进地钱愈伤组织生长。激素配比组合是0.5mg/L 2,4D+2mg/L NAA+2mg/L 6-BA适合于地钱细胞生长以及次生代谢生产。结论地钱愈伤组织的诱导成功和悬浮细胞培养条件的建立，为探索药用苔藓植物次生代谢产物生产提供新的途径以及对苔藓植物细胞规模化培养提供新的理论指导。

Abstract: To induce callus tissues and establish suspension culture of Marchantia polymorpha L. Methods MSK-2 and MS mediums were used to induce gametophyte into callus tissues, and fuzzy comprehensive evaluation method was used to assess the optimal phytohormone combination. ResultsBoth MSK-2 and MS (2mg/L 6-BA) medium could induce callus tissue formation, and the MSK-2 medium was superior to the MS medium for callus tissue induction. Light intensity（3?0008?000 lux） effectively promoted the callus tissue growth. Optimal phytohormone combination for cell growth and secondary metabolism production was 0.5mg/L 2, 4D+2mg/L NAA+2mg/L 6BA. Conclusion Successful induction of the callus tissues and establishment of suspension culture of Marchantia polymorpha L. provide a new pathway for exploring the pharmaceutical moss to produce secondary metabolism and a new theory guidance for suspension culture of moss cell in a large scale.

Key words: Bryophyte, Marchantia polymorpha L., Callus tissue, Cell culture

中图分类号:

Q813.1

尹德明,温学森,娄红祥. 地钱愈伤组织的诱导及其细胞培养条件的建立[J]. 山东大学学报(医学版), 2008, 46(4): 433-437.

YIN De-ming,WEN Xue-sen,LOU Hong-xiang. Initiation of callus tissues and establishment of suspension culture of Marchantia polymorpha L. cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(4): 433-437.

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