戊二醛鞣制驴心包与牛心包的体外力学性能比较

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (6): 42-.

戊二醛鞣制驴心包与牛心包的体外力学性能比较

陈善良1,许莉2，王雪梅2，刘羽曦3，厉泉2，张辉2，路平2，刘天起2

1.潍坊医学院, 山东潍坊 261053； 2.山东大学附属千佛山医院心外科, 山东省心脏移植与
材料工程技术研究中心, 济南 250014； 3.山东中医药大学基础医学院，济南 250355

收稿日期:2012-01-21 出版日期:2012-06-10 发布日期:2012-06-10
通讯作者: 刘天起(1959- )，男，教授，硕士生导师，主要从事心脏移植及生物瓣膜研究。 E-mail:tianqiliu@hotmail.com
作者简介:陈善良(1984- )，男，硕士研究生，主要从事心脏生物瓣膜研究
基金资助:
山东省科技攻关计划资助项目(2007GG10002003)

Comparison of mechanical properties between glutaraldehyde tanned
donkey pericardium and bovine pericardium in vitro

CHEN Shan-liang1， XU Li2， WANG Xue-mei2， LIU Yu-xi3，
LI Quan2， ZHANG Hui2， LU Ping2， LIU Tian-qi2

1． Weifang Medical College， Weifang 261053， Shandong， China;
2． Department of Cardiac Surgery， Shandong Provincial Qianfoshan Hospital Affiliated to Shandong University，
Shandong Heart Transplantation and Materials Engineering Technology Research Center， Jinan 250014， China;
3. The Basic Medical Sciences of Shandong University of Traditional Chinese Medicine, Jinan 250355， China

Received:2012-01-21 Online:2012-06-10 Published:2012-06-10

摘要/Abstract

摘要：

目的探讨驴心包在力学性能方面是否符合生物瓣膜的制备条件。方法用戊二醛鞣制脱细胞处理后的驴心包和牛心包进行对比研究。光镜下观察两组脱细胞效果。力学实验测定两组收缩温度、心包厚度、最大载荷、抗拉强度和撕裂强度。结果脱细胞处理后，光镜下见戊二醛鞣制的驴心包和牛心包无残存的细胞核,而未经脱细胞处理的驴心包和牛心包可见弥漫性细胞核存在,两种心包无明显差异；驴心包组收缩温度(87.5000±0.5916)℃,牛心包组收缩温度为(87.5600±0.3715)℃，两组差异无统计学意义(P>0.05)；取相同心尖部位的驴心包与牛心包比较，驴心包厚度薄，抗拉强度大(P<0.001)；两者的最大载荷和撕裂强度差异无统计学意义(P>0.05)。结论戊二醛鞣制的驴心包在力学性能方面适合制作心脏瓣膜材料。

关键词: 戊二醛；生物瓣膜；牛心包；驴心包

Abstract:

Objective To investigate donkey pericardial mechanical properties in biological valve preparation conditions. Methods Glutaraldehyde tanned acellular donkey pericardium was regarded as the experimental group, and glutaraldehyde tanned acellular bovine pericardium was regarded as the control group. The donkey pericardium and bovine pericardium were observed under a light microscope to identify the decellularized effect. The properties of temperature shrinkage, pericardial thickness, maximum load, tensile strength and tear strength were compared between the two groups. Results There were no residual nuclei in the decellularized donkey and bovine pericardium. There were many visible diffuse nuclei existing in the non-decellularized donkey and bovine pericardium. There was no difference between the two groups. The statistical analysis of shrinking temperature showed no significant difference between the experimental group (87.5000±0.5916)℃ and the control group(87.5600±0.3715)℃ (P>0.05 ). The donkey pericardium were significantly thinner in thickness and higher in tensile strength than the bovine pericardium (P<0.001). However, there were no differences between the two groups in temperature shrinkage, maximum load and tear strength (P>0.05). Conclusion Glutaraldehyde tanned donkey pericardium is suitable for the production of heart valve materials with mechanical properties.

Key words: Glutaraldehyde; Biological valve; Bovine pericardium; Donkey pericardium

中图分类号:

R318.08

陈善良1,许莉2，王雪梅2，刘羽曦3，厉泉2，张辉2，路平2，刘天起2. 戊二醛鞣制驴心包与牛心包的体外力学性能比较[J]. 山东大学学报(医学版), 2012, 50(6): 42-.

CHEN Shan-liang1， XU Li2， WANG Xue-mei2， LIU Yu-xi3， LI Quan2， ZHANG Hui2， LU Ping2， LIU Tian-qi2. Comparison of mechanical properties between glutaraldehyde tanned
donkey pericardium and bovine pericardium in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(6): 42-.

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