腺苷受体A3在特发性炎症性肌病中的表达

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (6): 105-.

腺苷受体A3在特发性炎症性肌病中的表达

遇晓1，于丕先2,赵丹丹3，刘付臣3，焉传祝3

山东大学 1.齐鲁医院风湿科, 济南 250012；2.附属济南市中心医院神经内科，济南 250012；
3.齐鲁医院神经内科神经肌肉病实验室，济南 250012

收稿日期:2012-02-21 出版日期:2012-06-10 发布日期:2012-06-10
通讯作者: 焉传祝(1964- )，男，教授，博士生导师，主要从事神经肌肉病的研究。 E-mail:czyan@yahoo.cn
作者简介:遇晓(1976- )，女，主治医师，博士研究生，主要从事炎症性肌病的研究。

A3 adenosine receptor expression in idopathic
inflammatory myopathies

YU Xiao1, YU Pi-xian2, ZHAO Dan-dan3, LIU Fu-chen3, YAN Chuan-zhu3

1. Department of Rheumatology, Qilu Hospital of Shandong University, Jinan 250012, China;
2. Department of Neurology, Jinan Central Hospital Affilicated to Shandong University, Jinan 250012, China;
3. Laboratory of Neuromuscular Disease, Department of Neurology, Qilu Hospital of
Shandong University, Jinan 250012, China

Received:2012-02-21 Online:2012-06-10 Published:2012-06-10

摘要/Abstract

摘要：

目的探讨腺苷受体A3(A3AR)在特发性炎症性肌病(IIMs)发病机制中的作用。方法收集IIMs患者骨骼肌活检组织标本30例，其中皮肌炎(DM)患者15例、多发性肌炎(PM)患者11例、包涵体肌炎(IBM)患者4例;正常对照标本12例。在冰冻切片上行组织化学染色和A3AR抗体免疫荧光染色。结果 A3AR抗体免疫荧光染色阳性见于骨骼肌2型肌纤维胞浆，1型肌纤维胞浆呈阴性；与正常对照比较，30例IIMs患者中22例2型肌纤维胞浆荧光增强。肌纤维膜荧光染色在正常对照呈阴性，而19例IIMs患者呈阳性。血管平滑肌和部分坏死肌纤维A3AR抗体免疫荧光染色呈强阳性。结论人骨骼肌2型肌纤维表达A3AR；特发性炎症性肌病患者A3AR表达增加，可能对肌纤维的修复和凋亡起双向调节作用。

关键词: 受体,嘌呤能P1；肌炎；活组织检查，肌肉

Abstract:

Objective To investigate the effects of A3 adenosine receptor(A3AR)expression in the pathogenesis of idopathic inflammatory myopathies( IIMs). Methods Muscle tissues from 30 cases of IIMs including 15 cases of dermatomyositis(DM), 11 cases of polymyositis(PM), 4 cases of inclusion body myositis(IBM) and 12 cases of normal controls were collected. Histochemical staining and immunofluorescent staining of the A3AR antibody were used to detect the existence of A3AR in the muscle biopsies． Results The cytosolic immunofluorescent staining of A3AR antibody was positive in the type Ⅱ muscle fibers, however, the staining was absent in the typeⅠmuscle fibers. 22 cases among the 30 cases of IIMs displayed a more intense cytosolic staining compared with that of the controls. Cell membrane fluorescent staining was positive in 19 cases of the IIMs, but it was negative in the controls. The highest intensity of the immunofluorescent staining for the A3AR antibody was localized in vascular smooth muscles and some necrotic fibers. Conclusions The A3AR expresses in human type Ⅱ skeletal muscle fibers. Expression of A3AR increases in idopathic inflammatory myopathies (IIMs) patients, and it may have a bidirectional regulation in the repairing and apoptosis of muscle fibers.

Key words: Receptors, purinergic P1; Myositis; Biopsy, muscle

中图分类号:

R392.31

遇晓1，于丕先2,赵丹丹3，刘付臣3，焉传祝3. 腺苷受体A3在特发性炎症性肌病中的表达[J]. 山东大学学报(医学版), 2012, 50(6): 105-.

YU Xiao1, YU Pi-xian2, ZHAO Dan-dan3, LIU Fu-chen3, YAN Chuan-zhu3. A3 adenosine receptor expression in idopathic
inflammatory myopathies[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(6): 105-.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed