TGF-β1对糖尿病大鼠深Ⅱ度烫伤创面愈合的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (7): 19-23.

TGF-β1对糖尿病大鼠深Ⅱ度烫伤创面愈合的影响

王文婷，单菲，王晓川，刘振中，张基勋，姜笃银

山东大学第二医院烧伤科，济南　250033

收稿日期:2010-12-05 出版日期:2011-07-10 发布日期:2011-07-10
通讯作者: 姜笃银(1963- )，男，博士，主任医师，主要从事烫伤创面修复的基础和临床研究。 E-mail:jdybs@vip.163.com
作者简介:王文婷(1984- )，女，硕士研究生，主要从事烫伤创面修复的基础和临床研究。
基金资助:
国家自然科学基金资助项目(30772258)

Effects of TGF-β1 on wound healing in diabetic rats with seconddegree scald

WANG Wen-ting, SHAN Fei, WANG Xiao-chuan, LIU Zhen-zhong, ZHANG Ji-xun, JIANG Du-yin

Department of Burn Surgery, The Second Hospital of Shandong University, Jinan 250033, China

Received:2010-12-05 Online:2011-07-10 Published:2011-07-10

摘要/Abstract

摘要：

目的观察转化生长因子β1 (TGFβ1) 在糖尿病大鼠深Ⅱ度烫伤创面组织中的表达，探讨外源性TGF-β1应用对创面愈合的影响。方法 18只SD大鼠随机分为单纯烫伤组(对照组)、糖尿病烫伤组(糖尿病组)和糖尿病烫伤TGF-β1干预组(用药组),每组各6只。糖尿病组和用药组大鼠经链脲佐菌素诱导建立糖尿病模型，再制备深Ⅱ度烫伤模型，用药组大鼠建模成功后给予创面下局部皮内注射TGF-β1。烫伤后1、3、5、7、14、21d记录创面未愈合面积并计算创面愈合率，组织学检查观察创面愈合情况，免疫组化检测创面组织TGF-β1表达。结果从烫伤后5d起，对照组和用药组创面愈合率较糖尿病组升高，差异有统计学意义(P<0.05)，对照组与用药组比较差异无统计学意义(P＞0.05)。对照组创面局部TGF-β1含量较糖尿病组多，差异有统计学意义(P<0.05)。结论糖尿病大鼠烫伤创面较正常大鼠烫伤创面愈合速度慢，与创面局部TGF-β1含量减少有关。创面局部应用TGF-β1有利于糖尿病大鼠烫伤创面愈合。

关键词: 转化生长因子-β1；糖尿病；创面愈合

Abstract:

Objective To observe expression of transforming growth factor β1(TGF-β1) in second-degree scald tissues in diabetic rats, and investigate the effect of TGF-β1 on the wound healing process. Methods Eighteen SD rats were randomly divided into 3 groups: the scald group (control group), the diabetic scald group (diabetic group) and the diabetic scald with TGF-β1 treatment group (TGFβ1 group), 6 rats in each group. Rats in the diabetic group and the TGF-β1 group were treated with streptozotocin to induce diabetes, and second-degree scald models were established. After model establishment, local intra-dermal injection of TGF-β1 into the wound was performed in the TGF-β1 group. The unhealed areas of wounds were recorded and wound healing rates were calculated 1, 3, 5, 7, 14 and 21 days after scald. The healing process was evaluated by histological examination, and expression of TGF-β1 was detected by the immunohistochemiscal technique. The data were statistically analyzed. Results Five days after scald, the healing rate in the control group and the TGF-β1 group was significantly higher than that in the diabetic group (P<0.05), while there was no significant difference between the control group and the TGF-β1group. Compared with the control group, expression of TGF-β1 was lower in scald tissues in the diabetic group (P<0.05). Conclusion The unfavorable healing process is related to decreased amount of TGF-β1 in diabetic wounds. Topical application of TGF-β1 can accelerate wound healing in diabetic rats.

Key words: Transforming growth factor β1; Diabetes mellitus; Wound healing

中图分类号:

R644

王文婷，单菲，王晓川，刘振中，张基勋，姜笃银. TGF-β1对糖尿病大鼠深Ⅱ度烫伤创面愈合的影响[J]. 山东大学学报(医学版), 2011, 49(7): 19-23.

WANG Wen-ting, SHAN Fei, WANG Xiao-chuan, LIU Zhen-zhong, ZHANG Ji-xun, JIANG Du-yin. Effects of TGF-β1 on wound healing in diabetic rats with seconddegree scald[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(7): 19-23.

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