促红细胞生成素对全脑缺血再灌注损伤大鼠血红素加氧酶-1表达的影响

山东大学学报(医学版)

促红细胞生成素对全脑缺血再灌注损伤大鼠血红素加氧酶-1表达的影响

黄涛¹，林晓英²，李芳邻¹，钟宁¹，刘运芳¹，孟晓慧¹，郑玉兰¹，宋文延¹

1. 山东大学医学院诊断学研究所，济南 250012； 2. 济南市中心医院神经内科，济南 250013

收稿日期:2007-09-19 修回日期:1900-01-01 出版日期:2008-03-16 发布日期:2008-03-16
通讯作者: 李芳邻

Effects of erythropoietin on heme oxygenase-1 expression in rats with globle ischemia-reperfusion injury

HUANG Tao¹，LIN Xiao-ying²，LI Fang-lin¹，ZHONG Ning¹，LIU Yun-fang¹，MENG Xiao-hui¹，ZHENG Yu-lan¹，SONG Wen-yan¹

1． Department of Diagnostics, School of Medicine, Shandong University， Jinan 250012, China;2. Department of Neurology, Jinan Central Hospital, Jinan 250013, China

Received:2007-09-19 Revised:1900-01-01 Online:2008-03-16 Published:2008-03-16
Contact: LI Fang-lin

摘要/Abstract

摘要： 目的探讨促红细胞生成素（EPO）对大鼠脑缺血再灌注后血红素加氧酶1（HO-1）表达的影响。方法随机将雄性SD大鼠分为缺血再灌注组、治疗组、假手术组。治疗组按照缺血再灌注后不同时间点给予EPO腹腔注射，假手术组不手术，于各时间点断头取脑，用逆转录-聚合酶链反应技术检测大鼠皮层HO-1 mRNA的表达。结果① 全脑缺血再灌注后HO-1 mRNA的表达发生动态变化，缺血后6h开始明显升高，24h达高峰，此后缓慢下降；② EPO能够显著上调全脑缺血后皮层HO-1 mRNA的表达,缺血再灌注后即注射EPO组与缺血再灌注组各时间点HO-1和β-actin吸光度的比值差异有统计学意义（P＜0.05 ）。结论EPO的神经保护作用可能部分通过上调HO-1的表达来实现。

Abstract: To explore the effect of erythropoietin（EPO）treatment on the expression of heme oxygenase-1（HO-1）mRNA in rats with globe ischemia-reperfusion injury. Methods The 4-vessel obstructive globe ischemic rat models were used in this study. The rats were randomly divided into three groups: the ischemia-reperfusion group, the experimental group and sham-operation group. Rats in the ischemiareperfusion group and the experimental group were subjected to EPO injection into the abdominal cavity after globe ischemia, however rats in the sham-operation group were not subjected to an operation. The expression of HO-1 mRNA was determined by RT-PCR. Results① After ischemia, the expression of HO1 mRMA was obviously changed based on time. ② The effect of EPO treatment on the expression of HO-1 mRNA in the cortex of this rat model was obvious. ConclusionThe protective effect of EPO for ischemiareperfusion injury is perhaps induced by up-regulating the expression of HO-1.

Key words: Heme oxygenase(Decyclizing), Erythropoietin, Reperfusion injury

中图分类号:

R743.33

黄涛,林晓英,李芳邻,钟宁,刘运芳,孟晓慧,郑玉兰,宋文延 . 促红细胞生成素对全脑缺血再灌注损伤大鼠血红素加氧酶-1表达的影响[J]. 山东大学学报(医学版), 2008, 46(3): 225-228.

HUANG Tao,LIN Xiao-ying,LI Fang-lin,ZHONG Ning,LIU Yun-fang,MENG Xiao-hui,ZHENG Yu-lan,SONG Wen-yan. Effects of erythropoietin on heme oxygenase-1 expression in rats with globle ischemia-reperfusion injury[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(3): 225-228.

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