利用网络数据库预测脂肪因子APLN、NUCB2、RARRES2在胶质母细胞瘤中的表达

doi:10.6040/j.issn.1671-7554.0.2019.426

摘要/Abstract

摘要： 目的探讨脂肪因子爱帕琳肽(APLN)、核连蛋白2(NUCB2)及维甲酸受体反应蛋白2(RARRES2)在胶质母细胞瘤(GBM)中的表达及意义。方法通过基因表达谱交互式分析网站(GEPIA)分析APLN、NUCB2及RARRES2 的mRNA在GBM组织及对照组织的表达,分析三者与血管内皮生长因子A(VEGFA)、CD34分子(CD34)及基质金属蛋白酶14(MMP14)的相关性;运用GEPIA和Survexpress分析三者与GBM预后的联系;使用癌细胞系百科全书(CCLE)分析APLN及其受体、NUCB2、RARRES2及其受体在4种胶质瘤细胞系中的表达。结果 APLN、NUCB2及RARRES2的mRNA在GBM患者组织中的表达均上调(P<0.05);APLN与VEGFA(R=0.52, P<0.05)、CD34(R=0.39, P<0.05)相关;NUCB2与MMP14相关(R=0.43,P<0.05);RARRES2的表达水平与GBM的预后呈负相关(GEPIA:Log rank P=0.027;Survexpress:Log rank P=0.007)。NUCB2在4种细胞系中均高表达。APLN受体在A172中表达最高;RARRES2受体在U87MG中表达最高。结论 APLN、NUCB2及RARRES2在GBM组织高表达,APLN与GBM血管生成、NUCB2与GBM侵袭及RARRES2与GBM预后相关。

关键词: 胶质母细胞瘤, 爱帕琳肽, 核连蛋白2, 维甲酸受体反应蛋白2, 数据分析

Abstract: Objective To investigate the expressions and significances of adipokines apelin(APLN), nucleobindin 2(NUCB2)and retinoic acid receptor responder protein 2(RARRES2)in glioblastoma multiforme(GBM). Methods The mRNA expressions of APLN, NUCB2 and RARRES2 in GBM tissues and control tissues, and the correlations between them and vascular endothelial growth factor A(VEGFA), CD34 and matrix metalloproteinase 14(MMP14)were determined with GEPIA. The relationship between APLN, NUCB2 and RARRES2 expressions and GBM prognosis was analyzed with GEPIA and Survexpress. The mRNA expressions of APLN and its receptor, NUCB2, RARRES2 and its receptors in 4 glioma cell lines were analyzed with cancer cell line encyclopedia(CCLE). Results The mRNA expressions of APLN, NUCB2 and RARRES2 were significantly up-regulated in GBM patients (P<0.05); APLN was associated with VEGFA (R=0.52, P<0.05) and CD34 (R=0.39, P<0.05); NUCB2 was associated with MMP14 (R=0.43, P<0.05); The expression of RARRES2 was negatively correlated with the prognosis of GBM(GEPIA: Log rank P=0.027; Survexpress: Log rank P=0.007). NUCB2 was highly expressed in all 4 cell lines. The APLN receptor was expressed the highest in A172 cells. The RARRES2 receptor was expressed the highest in U87MG cells. Conclusion APLN, NUCB2 and RARRES2 are highly expressed in GBM tissues. APLN is closely related to GBM angiogenesis. NUCB2 may link to GBM invasion and RARRES2 is associated with poor prognosis.

Key words: Glioblastoma multiforme, Apelin, Nucleobindin 2, Retinoic acid receptor responder protein 2, Data analysis

中图分类号:

R739.4

孔娟,张珍,郭伟, 韩萍. 利用网络数据库预测脂肪因子APLN、NUCB2、RARRES2在胶质母细胞瘤中的表达[J]. 山东大学学报 (医学版), 2019, 57(6): 61-67.

KONG Juan, ZHANG Zhen, GUO Wei, HAN Ping. Expressions of apelin, nucleobindin 2 and retinoic acid receptor responder protein 2 in glioblastoma based on network databases[J]. Journal of Shandong University (Health Sciences), 2019, 57(6): 61-67.

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