芳姜黄酮对SiHa细胞增殖、迁移及侵袭的影响

doi:10.6040/j.issn.1671-7554.0.2018.1443

摘要/Abstract

摘要： 目的研究芳姜黄酮对子宫颈癌SiHa细胞体外增殖、迁移与侵袭的作用,并初步探讨其机制。方法体外培养SiHa细胞,不同浓度芳姜黄酮(0、20、40、80 mg/L)作用于细胞后,采用CCK8法检测SiHa细胞体外增殖情况;吉姆萨染色显微镜观察SiHa细胞凋亡情况;细胞划痕实验和Transwell小室检测SiHa细胞体外迁移和侵袭能力;流式细胞术分析确定SiHa细胞的诱导凋亡率;Western blotting检测SiHa细胞MMP2、MMP9及p53蛋白表达。结果芳姜黄酮可抑制SiHa细胞的体外增殖(F浓度=162.419,P<0.001;F时间=99.442,P<0.001;F浓度×时间=2.111,P=0.095)、迁移(F浓度=432.158,P<0.001;F时间=528.845,P<0.001)和侵袭(F浓度=434.362,P<0.001),且与其浓度、作用时间呈正相关;流式细胞仪分析表明,芳姜黄酮可诱导SiHa细胞凋亡(F浓度=482.690,P<0.001),且与其浓度呈正相关;Western blotting检测结果表明,经芳姜黄酮处理后,凋亡相关蛋白p53的表达明显上调(F浓度=2.086,P=0.045)、侵袭和转移相关蛋白MMP2、MMP9的表达下调(F浓度=18.906,P=0.001;F浓度=9.847,P=0.005)。结论芳姜黄酮具有抑制SiHa细胞体外增殖及诱导细胞凋亡的能力,其机制可能与调节凋亡相关蛋白p53表达有关;芳姜黄酮可能通过调节侵袭和转移相关蛋白MMP2、MMP9的表达,进而抑制SiHa细胞迁移与侵袭。

关键词: 芳姜黄酮, SiHa细胞, 凋亡, 迁移, 侵袭

Abstract: Objective To investigate the effect of ar-turmerone on the proliferation, migration and invasion of cervical cancer SiHa cells in vitro, and to preliminarily explore its mechanism. Methods SiHa cells were incubated with different concentrations of ar-turmerone(0, 20, 40, 80 mg/L), and cell proliferation was measured by CCK8. The apoptosis was observed by giemsa staining. The effects of ar-turmerone on migration and invasion capacity of SiHa cells were detected by cell scratch assay and transwell. The apoptosis was analyzed by flow cytometry. The expressions of MMP2, MMP9 and p53 protein in SiHa cells were detected by Western blotting. Results Ar-turmerone dose-dependently inhibited SiHa cells proliferation(F_{concentration}=162.419, P<0.001; F_time=99.442, P<0.001; F_{concentration×time}=2.111, P=0.095), migration(F_{concentration}=432.158, P<0.001; F_time=528.845, P<0.001)and invasion(F_{concentration}=434.362, P<0.001). 山东大学学报 (医学版)57卷6期 -罗梦颖,等.芳姜黄酮对SiHa细胞增殖、迁移及侵袭的影响 \=-Flow cytometry analysis showed that ar-turmerone could induce the apoptosis of SiHa cells in a dose-dependent manner(F_{concentration}=482.690, P<0.001). Western blotting results showed that ar-turmerone treatment significantly up-regulated the expression of apoptosis-related proteins p53(F_{concentration}=2.086, P=0.045)and down-regulated the expressions of invasion and metastasis related proteins MMP2 and MMP9(F_{concentration}=18.906, P=0.001; F_{concentration}=9.847, P=0.005). Conclusion Ar-turmerone has the ability to inhibit the proliferation and induce apoptosis of SiHa cells in vitro, and the mechanism is related to the regulation of apoptosis related protein p53 expression. In addition, ar-turmerone can inhibit the migration and invasion of SiHa cells, possibly through the regulation of the expressions of MMP2 and MMP9 protein.

Key words: Ar-turmerone, SiHa cell, Apoptosis, Migration, Invasion

中图分类号:

R285.5

罗梦颖,吴蓓,王叶,骆衡,曹煜. 芳姜黄酮对SiHa细胞增殖、迁移及侵袭的影响[J]. 山东大学学报 (医学版), 2019, 57(6): 68-74.

LUO Mengying, WU Bei, WANG Ye, LUO Heng, CAO Yu. Effect of ar-turmerone on poliferation, migration and invasion of cervical squamous cancer cell line SiHa[J]. Journal of Shandong University (Health Sciences), 2019, 57(6): 68-74.

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