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山东大学学报(医学版) ›› 2017, Vol. 55 ›› Issue (1): 44-48.doi: 10.6040/j.issn.1671-7554.0.2016.942

• 临床医学 • 上一篇    下一篇

环氧化酶-2基因启动子区甲基化水平在子宫内膜异位症中的作用

陈琦1,李佳琪1,郭智彬2,贾惊宇3,何林生1   

  1. 1. 南昌大学第一附属医院妇产科;2. 江西省儿童医院中心实验室;3. 南昌大学第二附属医院骨科, 江西 南昌 330006
  • 收稿日期:2016-08-03 出版日期:2017-01-10 发布日期:2017-01-10
  • 通讯作者: 何林生. E-mail:whlsls@126.com E-mail:whlsls@126.com
  • 基金资助:
    国家自然科学基金(81560247);江西省教育厅科学技术研究项目(GJJ13123)

Role of methylation status of the cyclooxygenase-2 gene promoter in endometriosis

CHEN Qi1, LI Jiaqi1, GUO Zhibin2, JIA Jingyu3, HE Linsheng1   

  1. 1. Department of Obstetrics &
    Gynecology, the First Affiliated Hospital of Nanchang University;
    2. Deaprtment of Central Laboratory, Childrens Hospital of Jiangxi Province;
    3. Department of Orthopedics, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China
  • Received:2016-08-03 Online:2017-01-10 Published:2017-01-10

摘要: 目的 检测环氧化酶-2(COX-2)基因启动子区CRE位点甲基化状况,探讨其在子宫内膜异位症(EMs)发生中的作用。 方法 收集南昌大学第一附属医院40例子宫内膜异位症患者的子宫内膜作为病例组,同期40例正常子宫内膜作为对照组,采用焦磷酸盐测序技术检测病例组和对照组子宫内膜组织中COX-2基因启动子区CRE位点甲基化频率;采用实时定量PCR及免疫组化技术分别检测病例组和对照组子宫内膜组织中COX-2 mRNA及蛋白的表达;分析COX-2基因启动子区CRE位点甲基化频率与COX-2表达水平的相关性。 结果 对照组内膜组织COX-2基因启动子区CRE位点的甲基化频率(40.81%±6.10%)显著高于病例组(14.74%±2.84%),P<0.05;而对照组COX-2 mRNA表达水平(0.98±0.37)及蛋白表达水平(1.00±0.73)显著低于病例组的mRNA表达水平(2.63±0.44)及蛋白表达水平(2.74±1.18),P均<0.05。随着COX-2基因启动子区CRE位点甲基化频率升高,COX-2基因表达水平下降,二者呈负相关(P<0.05)。 结论 COX-2 基因启动子区CRE位点去甲基化与EMs患者在位内膜COX-2高表达相关,在EMs的发生、发展中具有重要作用,提示子宫内膜异位症是一种表观遗传性疾病。

关键词: 子宫内膜异位症, 环氧化酶-2, 甲基化, CRE位点

Abstract: Objective To study the methylation status of CRE sites in the cyclooxygenase-2(COX-2)gene promoter and its correlationship with endometriosis(EMs). Methods The endometrial tissues were collected from 40 women with laparoscopically confirmed EMs(case group), and 40 women without EMs(control group). The methylation status of COX-2 was examined by pyrosequencing. Quantitative real-time RT-PCR and immunohistochemistry were respectively performed to measure mRNA expression levels and protein levels of COX-2 in endometrial tissues. Finally, the correlation between COX-2 expression level and methylation frequencies of CRE sites were analyzed. Results The methylation frequencies of CRE sites in the COX-2 gene promoter was higher in the control group(40.81%±6.10%)than that in the case group(14.74%±2.84%), P<0.05. COX-2 mRNA levels(0.98±0.37)and protein levels(1.00±0.73)in the control group was significantly lower than the mRNA levels(2.63±0.44)and protein levels 山 东 大 学 学 报 (医 学 版)55卷1期 -陈琦,等.环氧化酶-2基因启动子区甲基化水平在子宫内膜异位症中的作用 \=-(2.74±1.18)in the case group, both P<0.05. The correlation between COX-2 expression levels and methylation frequencies of CRE sites in the COX-2 gene promoter was negative(P<0.05). Conclusion The hypomethylation of CRE sites within the promoter of COX-2 may be responsible for the elevated gene expression in eutopic endometrium of EMs.

Key words: Endometriosis, Cyclooxygenase-2, CRE sites, Methylation

中图分类号: 

  • R711.71
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