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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (3): 1-5.doi: 10.6040/j.issn.1671-7554.0.2014.943

• 基础医学 •    下一篇

全脑缺血大鼠脑组织MiR210和stat3的表达与HIF-1α的相关性

范少华1, 张轶超2, 袁晓东2, 毕景雯1, 张一辰2, 李云2   

  1. 1. 山东大学医学院, 山东 济南 250012;
    2. 山东大学附属济南市中心医院重症医学科, 山东 济南 250013
  • 收稿日期:2014-12-11 修回日期:2015-01-23 出版日期:2015-03-10 发布日期:2015-03-10
  • 通讯作者: 李云, E-mail:yunlee62@yahoo.com E-mail:yunlee62@yahoo.com
  • 基金资助:
    济南市科技发展计划(201401083)

Expressions of MiR210 and stat3 following global ischemia in rats and the potential relationship among MiR210, stat3 and HIF-1α

FAN Shaohua1, ZHANG Yichao2, YUAN Xiaodong2, BI Jingwen1, ZHANG Yichen2, LI Yun2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Critical Care Medicine, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Received:2014-12-11 Revised:2015-01-23 Online:2015-03-10 Published:2015-03-10

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摘要： 目的 研究全脑缺血再灌注损伤大鼠海马信号转导和转录激活因子3(stat3)及大脑皮层MiR210的表达变化, 探讨MiR210及stat3与缺氧诱导因子-1α(HIF-1α)之间的相关性及调节机制。方法 将66只SD大鼠随机分为假手术组、全脑缺血组(GI组)及stattic+全脑缺血组(stattic+GI组)。采用改良四血管阻断法制备大鼠全脑缺血模型, 免疫印迹法检测海马stat3、p-stat3及HIF-1α蛋白的表达, 实时定量PCR法检测大脑皮层MiR210及HIF-1α mRNA的变化。结果 与假手术组相比, GI组海马stat3、p-stat3及HIF-1α蛋白表达均明显增加(P<0.05), 大脑皮层MiR210及HIF-1α mRNA表达亦明显增加(P<0.05);与GI组相比, stattic+GI组海马p-stat3及HIF-1α蛋白表达均明显减少(P<0.05), 而stat3蛋白变化不明显(P>0.05);大脑皮层HIF-1α mRNA水平明显减少(P<0.05)。结论 全脑缺血再灌注损伤后, MiR210及stat3表达发生明显变化, 且两者与HIF-1α之间可能存在调控关系。

关键词: MiR210, 大鼠, 信号转导和转录激活因子3, 缺氧诱导因子-1α, 全脑缺血

Abstract: Objective To study the expression of signal transducer and activator of transcription 3 (stat3) in hippocampus and the expression of MiR210 in cerebral cortex following global ischemia in rats, and to explore the potential relationship among MiR210, stat3 and hypoxia inducible factor-1α (HIF-1α). Methods Sixty-six SD rats were randomly divided into three groups:sham-operated group, global ischemia group (GI group), and stattic+global ischemia group (stattic+GI group). The global ischemia model was induced by modified four-vessel occlusion method. Stat3, p-stat3 and HIF-1α protein expressions in hippocampus were detected by Western blotting. MiR210 and HIF-1α mRNA expressions in cerebral cortex were detected by Real-time PCR. Results Compared with sham-operated group, stat3, p-stat3 and HIF-1α protein expressions in hippocampus and MiR210 and HIF-1α mRNA expressions in cerebral cortex of GI group significantly increased (all P<0.05). Compared with GI group, p-stat3 and HIF-1α protein expressions in hippocampus of stattic+ GI group significantly decreased, stat3 protein expression showed no statistical difference (P>0.05), and HIF-1α mRNA expression in cerebral cortex signifcantly decreased (P<0.05). Conclusion Expressions of MiR210 and stat3 have a marketable change after global ischemia reperfusion, and regulatory relationships may exist among stat3, MiR210 and HIF-1α.

Key words: Rat, MiR210, Signal transducer and activator of transcripation 3, Global ischemia, Hypoxia inducible factor-1α

中图分类号: 

  • R743.31
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